https://scholars.lib.ntu.edu.tw/handle/123456789/500737
標題: | In vivo virtual biopsy of human skin by using noninvasive higher harmonic generation microscopy | 作者: | Chen, S.-Y. SHEE-UAN CHEN Wu, H.-Y. WEN-JENG LEE YI-HUA LIAO CHI-KUANG SUN |
公開日期: | 2010 | 卷: | 16 | 期: | 3 | 起(迄)頁: | 478-492 | 來源出版物: | IEEE Journal on Selected Topics in Quantum Electronics | 摘要: | Higher harmonic generation microscopy (HHGM), combining both second- and third-harmonic generation (SHG and THG) modalities, is a new paradigm for in vivo noninvasive virtual biopsy. With the ability to achieve noninvasiveness, high resolution, and high penetrability at the same time, HHGM is a promising tool for future noninvasive diagnosis of skin diseases. In this paper, we report our preliminary pilot clinical trial results on in vivo virtual biopsy of human skin by using HHGM. In vivo virtual biopsy imaging has been performed on 21 volunteers inside and outside forearm skin along with the damage evaluation. Together with an embryo viability study, our results not only indicate a superior viability performance of the developed system, but also a much improved penetrability in different skin types. Ex vivo studies further confirm the capability of the developed virtual biopsy system to pathohistologically distinguish different skin diseases. Our in vivo HHGM biopsy study of human skin with different colors also reveals the central role of melanin in the epi-THG resonance enhancement and attenuation. With a unique capability to molecular image the melanin distribution, epi-THG microscopy is also highly valuable for diagnosing and screening early melanocytic lesions. ? 2006 IEEE. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/500737 | DOI: | 10.1109/JSTQE.2009.2031987 | SDG/關鍵字: | Biomedical optical imaging; Clinical trial; Damage evaluation; Ex-vivo; Harmonic generation microscopy; High resolution; Higher harmonics; Human skin; In-vivo; Melanocytic lesion; Molecular images; Non-invasive diagnosis; Resonance enhancement; Skin disease; Third-harmonic generation; Biopsy; Dermatology; Harmonic analysis; Harmonic generation; Nonlinear optics; Optical image storage; Optical materials; Virtual reality |
顯示於: | 電機工程學系 |
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