https://scholars.lib.ntu.edu.tw/handle/123456789/501319
Title: | Escherichia coli fiber sensors using concentrated dielectrophoretic force with optical defocusing method | Authors: | Tai, Y.-H. Lee, C.-W. Chang, D.-M. Lai, Y.-S. DING-WEI HUANG |
Keywords: | defocused image; dielectrophoretic force; E. coli; fiber tip; refractive index detection | Issue Date: | 2018 | Journal Volume: | 3 | Journal Issue: | 6 | Start page/Pages: | 1196-1202 | Source: | ACS Sensors | Abstract: | A sensitive tapered optical fiber tip combined with dielectrophoretic (DEP) trapping was used for rapid and label-free detection of bacteria in water. The angular spectrum of the optical field at the fiber tip was changed with the surrounding refractive index (RI). By measuring far-field intensity change at the defocus plane, the intensity sensitivity was up to 95 200%/RIU (RI unit), and the detection limit was 5.2 × 10-6 RIU at 0.5% intensity stability. By applying an AC voltage to a Ti/Al coated fiber tip and an indium-tin-oxide glass, the DEP force effectively trapped the Escherichia coli ( E. coli) near the fiber tip. Those bacteria can be directly measured from optical intensity change due to the increase of surrounding RI. By immobilizing the antibody on the Ti/Al fiber tip, the tests for specific K12 bacteria and nonspecific BL21 bacteria verified the specificity. The antibody-immobilized Ti/Al coated fiber tip with DEP trapping can detect bacteria at a concentration about 100 CFU/mL. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/501319 https://www.scopus.com/inward/record.uri?eid=2-s2.0-85057401899&doi=10.1021%2facssensors.8b00258&partnerID=40&md5=6d2b70118184097ba9d979991e1dbdee |
DOI: | 10.1021/acssensors.8b00258 | SDG/Keyword: | aluminum; glass fiber; titanium; chemistry; electrophoresis; Escherichia coli; genetic procedures; isolation and purification; Aluminum; Biosensing Techniques; Electrophoresis; Escherichia coli; Optical Fibers; Titanium |
Appears in Collections: | 電機工程學系 |
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