https://scholars.lib.ntu.edu.tw/handle/123456789/508186
標題: | Decreased T helper 17 cells in tuberculosis is associated with increased percentages of programmed death ligand 1, T helper 2 and regulatory T cells | 作者: | CHIN-CHUNG SHU Wu M.-F. JANN-YUAN WANG HSIN-CHIH LAI LI-NA LEE BOR-LUEN CHIANG CHONG-JEN YU |
公開日期: | 2017 | 卷: | 18 | 期: | 1 | 起(迄)頁: | 128 | 來源出版物: | Respiratory Research | 摘要: | Background: Tuberculosis (TB) is one of the most common infectious diseases worldwide. During active tuberculosis, T helper (Th) 17 cells are decreased, however the association with inhibitory immune regulation is unclear. Methods: We enrolled 27 patients with TB and 20 age- and sex-matched controls and studies their lymphocyte status. Peripheral blood lymphocytes were isolated and programmed death-1 (PD-1) and programmed death ligand 1 (PD-L1) were measured on Th17 cells by using flow cytometry after the cells were stimulated with phorbol 12-myristate 13-acetate and ionomycin for 6 h. In addition, Th2 and regulatory T cells were measured and analyzed. Results: The TB group had lower levels of Th17 cells but higher levels of Th2 and Treg cells than the controls. In Th17 cells, the percentage of PD-L1 was higher in the TB group than that in the controls. In Th2 and Treg cells, the percentage of cytotoxic T-lymphocyte associated protein 4 (CTLA-4) was lower in the TB group and PD-1 was higher in Treg cells in the TB group. In the patients with extra-pulmonary TB, levels of Th1, Th2 and T17 cells were lower than those with pulmonary TB. The percentage of PD-1 on Th1 lymphocytes positively correlated with radiographic score. Conclusions: Lower level of Th17 in TB patients may be associated with increased percentage of PD-L1 and increasing levels of Th2 and Treg cells which influenced by CTLA-4. ? 2017 The Author(s). |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/508186 | ISSN: | 1465-9921 | DOI: | 10.1186/s12931-017-0580-3 | SDG/關鍵字: | cytotoxic T lymphocyte antigen 4; programmed death 1 ligand 1; programmed death 1 receptor; tuberculostatic agent; CD274 protein, human; programmed death 1 ligand 1; adult; age; Article; clinical article; controlled study; extrapulmonary tuberculosis; female; health status; human; human cell; lung tuberculosis; lymphocyte count; male; Mycobacterium tuberculosis; nonhuman; peripheral lymphocyte; radiodiagnosis; radiographic score; regulatory T lymphocyte; scoring system; sex; T lymphocyte activation; Th17 cell; Th2 cell; aged; blood; immunology; isolation and purification; metabolism; middle aged; prospective study; regulatory T lymphocyte; Th17 cell; Th2 cell; tuberculosis; Adult; Aged; B7-H1 Antigen; Female; Humans; Male; Middle Aged; Mycobacterium tuberculosis; Prospective Studies; T-Lymphocytes, Regulatory; Th17 Cells; Th2 Cells; Tuberculosis |
顯示於: | 醫學檢驗暨生物技術學系 |
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