https://scholars.lib.ntu.edu.tw/handle/123456789/508271
Title: | Surface plasmon resonance biochips for tuberculosis bacillus detection | Authors: | Huang J.-G. Hung C.-C.K. HSIN-CHIH LAI Lee C.-K. SHI-MING LIN Feng P. CHII-WANN LIN |
Issue Date: | 2004 | Journal Volume: | 26 IV | Start page/Pages: | 2553-2556 | Source: | Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings | Abstract: | Tuberculosis is one of notifiable infectious diseases which may cause serious epidemic problems. Traditional diagnostic techniques include acid- fast stain, bacteria culture, metabolic monitoring have some disadvantages such as low sensitivity, time consumption and less specificity. In this study we proposed a new diagnostic technique based on the protein chips concept which detected by SPR phenomena. It was found that the resonance angle (?51.86°) shifted slightly toward right with antibody concentration of 10X, 30X, 100X, and 300X dilution under optimal concentration of immobilized TB antigen W38 (MW 41.5 kDa, 50 μg/ml). While for the W06 (MW 14 kDa, 50 μg/ml), the resonance angle was around 50.13° and shifted with the same trend as W38 did. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/508271 | ISSN: | 0589-1019 | SDG/Keyword: | Antibodies; Bacteria; Concentration (process); Diseases; Molecular biology; Monolayers; Photoresists; Proteins; Biochips; Glass substartes; Protein chips; Tuberculosis; Surface plasmon resonance |
Appears in Collections: | 醫學檢驗暨生物技術學系 |
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