https://scholars.lib.ntu.edu.tw/handle/123456789/524903
標題: | Super-resolution architecture of mammalian centriole distal appendages reveals distinct blade and matrix functional components | 作者: | TUNG-LIN YANG Chong, W.M. Wang, W.-J. Mazo, G. Tanos, B. Chen, Z. Tran, T.M.N. Chen, Y.D. Weng, R.R. Huang, C.-E. Jane, W.-N. Tsou, M.F.B. Liao, J.C. |
公開日期: | 2018 | 出版社: | Scopus | 卷: | 9 | 期: | 1 | 起(迄)頁: | 263-275 | 來源出版物: | Nature Communications | 摘要: | Distal appendages (DAPs) are nanoscale, pinwheel-like structures protruding from the distal end of the centriole that mediate membrane docking during ciliogenesis, marking the cilia base around the ciliary gate. Here we determine a super-resolved multiplex of 16 centriole-distal-end components. Surprisingly, rather than pinwheels, intact DAPs exhibit a cone-shaped architecture with components filling the space between each pinwheel blade, a new structural element we term the distal appendage matrix (DAM). Specifically, CEP83, CEP89, SCLT1, and CEP164 form the backbone of pinwheel blades, with CEP83 confined at the root and CEP164 extending to the tip near the membrane-docking site. By contrast, FBF1 marks the distal end of the DAM near the ciliary membrane. Strikingly, unlike CEP164, which is essential for ciliogenesis, FBF1 is required for ciliary gating of transmembrane proteins, revealing DAPs as an essential component of the ciliary gate. Our findings redefine both the structure and function of DAPs. © 2018 The Author(s). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85047631174&doi=10.1038%2fs41467-018-04469-1&partnerID=40&md5=04a3db09163d53b99d176377c943cb4f https://scholars.lib.ntu.edu.tw/handle/123456789/524903 |
ISSN: | 20411723 | DOI: | 10.1038/s41467-018-04469-1 | SDG/關鍵字: | cell membrane protein; protein cep164; protein cep83; protein cep89; protein dap; protein fbf1; protein sclt1; protein sstr3; Smoothened protein; unclassified drug; cell cycle protein; CEP164 protein, human; CEP83 protein, human; CEP89 protein, human; FBF1 protein, human; microtubule associated protein; microtubule protein; SCLT1 protein, human; signal transducing adaptor protein; sodium channel; anatomy; electronic equipment; equipment component; mammal; matrix; membrane; nanoparticle; Article; cell membrane; centriole; cilium; distal appendage matrix; extracellular matrix; human; human cell; molecular docking; cell line; centriole; chemistry; cilium; CRISPR Cas system; epithelium cell; gene editing; gene expression; genetics; HEK293 cell line; metabolism; molecular imaging; protein multimerization; retinal pigment epithelium; ultrastructure; Mammalia; Adaptor Proteins, Signal Transducing; Cell Cycle Proteins; Cell Line; Centrioles; Cilia; CRISPR-Cas Systems; Epithelial Cells; Gene Editing; Gene Expression; HEK293 Cells; Humans; Microtubule Proteins; Microtubule-Associated Proteins; Molecular Imaging; Protein Multimerization; Retinal Pigment Epithelium; Sodium Channels |
顯示於: | 電機工程學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。