https://scholars.lib.ntu.edu.tw/handle/123456789/527085
標題: | The levels of CD4+CD25+ regulatory T cells in paediatric patients with allergic rhinitis and bronchial asthma | 作者: | Lee J.-H. HSIN-HUI YU LI-CHIEH WANG YAO-HSU YANG YU-TSAN LIN BOR-LUEN CHIANG |
公開日期: | 2007 | 卷: | 148 | 期: | 1 | 起(迄)頁: | 53-63 | 來源出版物: | Clinical and Experimental Immunology | 摘要: | Our purpose was to determine whether numbers of CD4+CD25 + T [T regulatory (Treg)] cells and mRNA expression of functional molecules of Treg are related to airway allergy and disease severity in 51 paediatric patients with allergic rhinitis or bronchial asthma and 47 healthy controls. Surface markers were evaluated with flow cytometry, and mRNA was determined with real-time polymerase chain reaction. Children with allergic disease had fewer CD4+CD25+ T cells (8.49% ± 2.41% versus 9.58% ± 2.43%, P < 0.05) and CD4 +CD25hi T cells (1.32% ± 0.68% versus 1.70% ± 0.68%, P < 0.01) than control subjects. Numbers of CD4 +CD25+ and CD4+CD25hi T lymphocytes were higher in children with persistent allergic rhinitis and/or moderate-severe bronchial asthma than in those with respective milder disease. The number of Treg cells was correlated positively with total immunoglobulin E level. The mRNA expression of forkhead box P3 (FoxP3) was increased in moderate-severe versus mild asthma (2.93 ± 0.38 versus 1.60 ± 0.31, P < 0.01). Patients with moderate-severe bronchial asthma also had increased mRNA expression of interleukin (IL)-10 compared with patients with mild asthma (15.24 ± 4.07 versus 3.77 ± 2.18, P < 0.01). The suppressive function of Treg cells from patients with more severe asthma was competent in vitro. On average, decreased numbers of Treg cells in children with allergic airway disease might represent a defect of the Treg population. With increased expression of FoxP3 and IL-10 in Treg from patients with relatively severe allergic disease, adaptive and functional Treg might be generated in response to aggravated atopy and disease severity. ? 2007 British Society for Immunology. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-33847624061&doi=10.1111%2fj.1365-2249.2007.03329.x&partnerID=40&md5=ad2adc2f1e4b54337218475651dfb718 https://scholars.lib.ntu.edu.tw/handle/123456789/527085 |
ISSN: | 0009-9104 | DOI: | 10.1111/j.1365-2249.2007.03329.x | SDG/關鍵字: | immunoglobulin E; interleukin 10; messenger RNA; transcription factor FOXP3; allergic disease; allergic rhinitis; article; asthma; CD4+ CD25+ T lymphocyte; child; controlled study; cytokine release; disease severity; female; flow cytometry; human; human cell; immunoglobulin blood level; lymphocyte count; lymphocyte function; lymphocyte subpopulation; major clinical study; male; priority journal; protein expression; real time polymerase chain reaction; regulatory T lymphocyte; Antigens, CD; Antigens, Differentiation; Asthma; Cell Proliferation; Cells, Cultured; Child; Child, Preschool; Female; Forkhead Transcription Factors; Gene Expression; Humans; Immune Tolerance; Immunoglobulin E; Interleukin-10; Interleukin-2 Receptor alpha Subunit; Lymphocyte Count; Male; Polymerase Chain Reaction; Receptors, Nerve Growth Factor; Receptors, Tumor Necrosis Factor; Rhinitis; RNA, Messenger; Severity of Illness Index; T-Lymphocytes, Regulatory; Transforming Growth Factor beta |
顯示於: | 醫學系 |
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