https://scholars.lib.ntu.edu.tw/handle/123456789/535203
標題: | Molecular epidemiology, antimicrobial susceptibility and carbapenemase resistance determinants among Acinetobacter baumannii clinical isolates in Taiwan | 作者: | YU-CHUNG CHUANG WANG-HUEI SHENG Lauderdale T.-L. Li S.-Y. JANN-TAY WANG YEE-CHUN CHEN SHAN-CHWEN CHANG |
公開日期: | 2014 | 出版社: | Elsevier Ltd | 卷: | 47 | 期: | 4 | 起(迄)頁: | 324-332 | 來源出版物: | Journal of Microbiology, Immunology and Infection | 摘要: | Background: Emerging carbapenem resistance among Acinetobacter baumannii clinical isolates is a worldwide problem. Infections caused by A. baumannii are increasing and demonstrate high mortality rates. This study aimed to establish a nationwide surveillance of antimicrobial susceptibility, carbapenemase genes, and clonal relationships of A. baumannii clinical isolates in Taiwan. Methods: Clinical isolates of Acinetobacter calcoaceticus-A. baumannii (ACB) complex collected by the Taiwan Surveillance of Antimicrobial Resistance-V program between July 2006 and September 2006 were used in this study. Genospecies identification was verified by 16S-23S rRNA intergenic-spacer sequences. Carbapenemase genes were detected by polymerase chain reaction. Pulsed-field gel electrophoresis and multilocus sequence typing (MLST) was applied for identification of clonal relationships. Results: Among the 151 ACB-complex isolates collected, 134 (88.7%) were A. baumannii, 12 (8.0%) were A. pittii, and five (3.3%) were A. nosocomialis. A. baumannii isolates showed higher resistance rates to ciprofloxacin, amikacin, and ampicillin/sulbactam than A. pittii or A. nosocomialis (all p<0.001). The most commonly detected carbapenemase genes were blaOxA-51 (n=135), followed by blaOxA-24 (n=4), blaOxA-23 (n=2), and blaOxA-58 (n=1). Three major A. baumannii clones were found throughout Taiwan, and showed significantly higher resistance rates to ciprofloxacin, amikacin, and ampicillin/sulbactam than the other A. baumannii isolates (100% vs. 68.7%, p<0.001; 98.4% vs. 61.5%, p<0.001; and 66.7% vs. 39.8%, p=0.004; respectively). MLST showed that these major clones were sequence type 2 and belonged to international clonal complex 2. Conclusion: Our results demonstrate clonal spreading of A. baumannii in Taiwan hospitals and that these clones were more resistant to many antimicrobial agents. Efforts to prevent and control A. baumannii colonization/infections and prudent use of antibiotics to reduce antimicrobial selective pressure should be emphasized. ? 2013. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84904498158&doi=10.1016%2fj.jmii.2013.03.008&partnerID=40&md5=4d07bce2a661bb5c990fea32a5b2f25a https://scholars.lib.ntu.edu.tw/handle/123456789/535203 |
ISSN: | 1684-1182 | DOI: | 10.1016/j.jmii.2013.03.008 | SDG/關鍵字: | amikacin; antibiotic agent; carbapenem; carbapenemase; ciprofloxacin; colistin; imipenem; RNA 16S; RNA 23S; sultamicillin; tigecycline; antiinfective agent; carbapenem derivative; Acinetobacter baumannii; Acinetobacter calcoaceticus; Acinetobacter nosocomialis; Acinetobacter pittii; antibiotic resistance; antibiotic sensitivity; article; bacterium isolate; minimum inhibitory concentration; molecular epidemiology; multilocus sequence typing; nonhuman; polymerase chain reaction; pulsed field gel electrophoresis; Taiwan; Acinetobacter baumannii; Acinetobacter Infections; cluster analysis; drug effects; genetics; genotype; health survey; human; isolation and purification; microbial sensitivity test; microbiology; Acinetobacter baumannii; Acinetobacter Infections; Anti-Bacterial Agents; beta-Lactam Resistance; Carbapenems; Cluster Analysis; Electrophoresis, Gel, Pulsed-Field; Genotype; Humans; Microbial Sensitivity Tests; Multilocus Sequence Typing; Public Health Surveillance; Taiwan |
顯示於: | 醫學系 |
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