https://scholars.lib.ntu.edu.tw/handle/123456789/553199
Title: | Induced interleukin-8 expression in gliomas by tumor-associated macrophages | Authors: | Hong T.-M. LEE-JENE TENG CHIA-TUNG SHUN Peng M.-C. JUI-CHANG TSAI |
Issue Date: | 2009 | Journal Volume: | 93 | Journal Issue: | 3 | Start page/Pages: | 289-301 | Source: | Journal of Neuro-Oncology | Abstract: | The tumor microenvironment affects tumor initiation, progression, and metastasis. However, it is still not clear how stromal cells interact with the tumor cells. By using a cytokine array immunoblot assay, we showed that interleukin (IL)-8, IL-6, and RANTES (regulated upon activation normal T-cell expressed and secreted) proteins were up-regulated in GBM8401 glioma cells after coculture with human THP-1-derived macrophages. IL-8 is a chemokine with leukocyte chemotactic, tumorigenic, and proangiogenic properties. To evaluate the correlation of IL-8 expression with tumor-associated macrophages and angiogenesis, 43 glioma specimens were studied. The results showed that the IL-8 mRNA expression and microvessel count in glioma surgical specimens correlated positively with the density of tumor-associated macrophages. We further showed that IL-8 mRNA expression in GBM8401 cells increased dramatically, by 28-210-fold, after being cocultured with macrophages. This increase could also be induced by macrophage-conditioned medium, tumor necrosis factor-α, IL-1α, and IL-1β, and could be suppressed by anti-inflammatory agents including pyrrolidine dithiocarbamate, pentoxifylline, or dexamethasone. These findings imply that macrophage infiltration may be the common feature shared by cancer and inflammation, and macrophages could play a role in promoting glioma growth and angiogenesis by inducing IL-8 expression in glioma cells via inflammatory stimuli or the nuclear factor kappa B pathway. ? Springer Science+Business Media, LLC. 2009. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/553199 | ISSN: | 0167-594X | DOI: | 10.1007/s11060-008-9786-z | SDG/Keyword: | dexamethasone; immunoglobulin enhancer binding protein; interleukin 6; interleukin 8; messenger RNA; pentoxifylline; pyrrolidine dithiocarbamate; RANTES; recombinant interleukin 1alpha; recombinant interleukin 1beta; recombinant tumor necrosis factor alpha; angiogenesis; article; cell function; cell infiltration; coculture; controlled study; correlation analysis; gene expression; gene overexpression; glioma; human; human cell; microvasculature; pathogenesis; tumor associated leukocyte; upregulation; Brain Neoplasms; Chemokine CCL5; Coculture Techniques; Culture Media, Conditioned; Fluorescent Antibody Technique; Glioma; Humans; Immunohistochemistry; Interleukin-6; Interleukin-8; Macrophages; Microscopy, Confocal; Neovascularization, Pathologic; NF-kappa B; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Up-Regulation |
Appears in Collections: | 法醫學科所 |
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