https://scholars.lib.ntu.edu.tw/handle/123456789/557541
標題: | Establishment and characterization of NS3 protein-specific T-cell clones from a patient with chronic hepatitis C | 作者: | BOR-LUEN CHIANG PEI-MING YANG Hwang L.-H. Wang J.-M. Kao S.-F. Pan C.-H. Chi W.-K. PEI-JER CHEN DING-SHINN CHEN |
公開日期: | 1998 | 出版社: | BioMed Central Ltd. | 卷: | 5 | 期: | 4 | 起(迄)頁: | 290-296 | 來源出版物: | Journal of Biomedical Science | 摘要: | Our previous study showed dominant proliferative response of peripheral mononuclear cells to hepatitis C virus (HCV) nonstructural (NS-3) (T9, from aa 1188 to 1493) in chronically infected patients. Six T9-specific T-cell clones derived in an HCV patient were established and studied for the antigen specificity and the ability of augmentation of in vitro antibody production. All these cloned T-cell lines responded exclusively to T9 antigen and could help autologous B cells in producing anti-T9 antibody in vitro. Cytokine mRNAs of these T cells was detected by polymerase chain reaction and predominant IL-2 and IFN-γ production was noted. In addition, further elucidation of T-cell antigenic determinant and MHC restriction suggested that these T-cell clones recognized at least two different T-cell antigenic determinants within the NS-3 region in an HLA DQ2-restricted manner. We believe characterization of HCV-specific T-cell responses, especially T-cell epitope mapping and cytokine production pattern, may shed light on further understanding the pathogenic mechanism and designing therapy for HCV infection. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84984585951&doi=10.1007%2fBF02255861&partnerID=40&md5=c507d405618e6b754b8c6270c4dbb491 https://scholars.lib.ntu.edu.tw/handle/123456789/557541 |
ISSN: | 1021-7770 | DOI: | 10.1007/BF02255861 | SDG/關鍵字: | adult; antigenicity; article; case report; cellular immunity; chronic hepatitis; hepatitis C; Hepatitis C virus; human; male; pathogenesis; priority journal; T lymphocyte |
顯示於: | 醫學系 |
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