https://scholars.lib.ntu.edu.tw/handle/123456789/567724
標題: | A Polysaccharide Purified From Ganoderma lucidum Acts as a Potent Mucosal Adjuvant That Promotes Protective Immunity Against the Lethal Challenge With Enterovirus A71 | 作者: | Lin Y.-L. Shih C. Cheng P.-Y. Chin C.-L. Liou A.-T. Lee P.-Y. BOR-LUEN CHIANG |
關鍵字: | adjuvant; Enterovirus A71; IFN-γ; IL-17; intranasal; mucosal vaccine; polysaccharide from Ganoderma lucidum | 公開日期: | 2020 | 出版社: | Frontiers Media S.A. | 卷: | 11 | 起(迄)頁: | 561758 | 來源出版物: | Frontiers in Immunology | 摘要: | Enterovirus A71 (EV-A71), the pathogen responsible for the seasonal hand-foot-and-mouth epidemics, can cause significant mortality in infants and young children. The vaccine against EV-A71 could potentially prevent virus-induced neurological complications and mortalities occurring due to the high risk of poliomyelitis-like paralysis and fatal encephalitis. It is known that polysaccharide purified from Ganoderma lucidum (PS-G) can effectively modulate immune function. Here, we used PS-G as an adjuvant with the EV-A71 mucosal vaccine and studied its effects. Our data showed that PS-G-adjuvanted EV-A71 generated significantly better IgA and IgG in the serum, saliva, nasal wash, bronchoalveolar lavage fluid (BALF), and feces. More importantly, these antibodies could neutralize the infectivity of EV-A71 (C2 genotype) and cross-neutralize the B4, B5, and C4 genotypes of EV-A71. In addition, more EV-A71-specific IgA- and IgG- secreting cells were observed with the used of a combination of EV-A71 and PS-G. Furthermore, T-cell proliferative responses and IFN-γ and IL-17 secretions levels were notably increased in splenocytes when the EV-A71 vaccine contained PS-G. We also found that levels of IFN-γ and IL-17 released in Peyer’s patch cells were significantly increased in EV-A71, after it was combined with PS-G. We further demonstrated that both CD4+ and CD8+ T cells were able to generate IFN-γ and IL-17 in the spleen. An easy-accessed model of hybrid hSCARB2+/+/stat-1–/– mice was used for EV-A71 infection and pathogenesis. We infected the mouse model with EV?A71, which was premixed with mouse sera immunized with the EV-A71 vaccine with or without PS-G. Indeed, in the EV-A71 + PS-G group, the levels of VP1-specific RNA sequences in the brain, spinal cord, and muscle decreased significantly. Finally, hSCARB2-Tg mice immunized via the intranasal route with the PS-G-adjuvanted EV-A71 vaccine resisted a subsequent lethal oral EV-A71 challenge. Taken together, these results demonstrated that PS-G could potentially be used as an adjuvant for the EV-A71 mucosal vaccine. ? Copyright ? 2020 Lin, Shih, Cheng, Chin, Liou, Lee and Chiang. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85092576960&doi=10.3389%2ffimmu.2020.561758&partnerID=40&md5=ddd3a89946ec53c89ae1552a38eb6552 https://scholars.lib.ntu.edu.tw/handle/123456789/567724 |
ISSN: | 1664-3224 | DOI: | 10.3389/fimmu.2020.561758 | SDG/關鍵字: | adjuvant; diethylaminoethyl cellulose; formaldehyde; gamma interferon; immunoglobulin G; immunoglobulin G antibody; interleukin 10; interleukin 4; ionomycin; monensin; pilocarpine; tumor necrosis factor; virus vaccine; fungal polysaccharide; immunoglobulin A; immunological adjuvant; neutralizing antibody; virus antibody; virus vaccine; adult; animal experiment; animal model; antibody response; Article; bronchoalveolar lavage fluid; CD8+ T lymphocyte; cell proliferation; cell viability; centrifugation; controlled study; cytotoxicity; Enterovirus A71; enzyme linked immunosorbent assay; epidemic; female; flow cytometry; Ganoderma lucidum; gene expression; genotype; immune response; immunization; liquid chromatography; lung lavage; mortality rate; mouse; nonhuman; poliomyelitis; protein expression; regulatory T lymphocyte; T lymphocyte; vaccination; virus load; animal; blood; C57BL mouse; CD4+ T lymphocyte; chemistry; disease model; Enterovirus A; Enterovirus infection; fruiting body; Ganoderma lucidum; immunology; intranasal drug administration; knockout mouse; procedures; vaccine immunogenicity; virology; Adjuvants, Immunologic; Administration, Intranasal; Animals; Antibodies, Neutralizing; Antibodies, Viral; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; Disease Models, Animal; Enterovirus A, Human; Enterovirus Infections; Female; Fruiting Bodies, Fungal; Fungal Polysaccharides; Immunogenicity, Vaccine; Immunoglobulin A; Immunoglobulin G; Mice; Mice, Inbred C57BL; Mice, Knockout; Reishi; Vaccination; Viral Vaccines |
顯示於: | 醫學系 |
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