https://scholars.lib.ntu.edu.tw/handle/123456789/567778
標題: | Upregulated thymic stromal lymphopoietin receptor expression in children with asthma | 作者: | Lin S.-C. Huang J.-J. Wang J.-Y. Chuang H.-C. BOR-LUEN CHIANG Ye Y.-L. |
關鍵字: | Allergy; Asthma; IL-7Rα; Mite-sensitive; TSLPR | 公開日期: | 2016 | 出版社: | Blackwell Publishing Ltd | 卷: | 46 | 期: | 6 | 起(迄)頁: | 511-519 | 來源出版物: | European Journal of Clinical Investigation | 摘要: | Background: Thymic stromal lymphopoietin (TSLP) plays an important role in pathogenesis in patients with asthma. However, the role of thymic stromal lymphopoietin receptor (TSLPR) and correlation with IL-7Rα and clinical severity in asthmatic or nonasthmatic children remain unclear. We investigated TSLPR and IL-7Rα mRΝΑ levels in asthma and nonasthma and assessed TSLPR expression in children who were sensitive to mites. Materials and methods: We enrolled asthmatic and nonasthmatic children. To minimize the influence of allergy, we also divided participants into following 4 groups: nonallergic and nonasthmatic group (NN) (healthy children), allergic but nonasthmatic group (AN), nonallergic but asthmatic group (NA) and allergic asthmatic group (AA). We drew blood samples to check total IgE, allergen-specific IgE and TSLP and measured the expression of the TSLPR and IL-7Rα genes using reverse-transcription polymerase chain reaction (RT-PCR) and real-time PCR. Asthma symptom score was also recorded. Results: Thymic stromal lymphopoietin and TSLPR levels were found to be significantly higher in asthmatic than in nonasthmatic children. The levels of TSLP were found to be significantly different between AA and NN groups (P < 0·05). TSLPR expression in NA and AA groups was found to be significantly higher than in NN group (P < 0·05). TSLPR did not differ significantly between NA and AA groups. The TSLPR expression correlated strongly with IL-7Rα and weakly with mite-specific IgE. Clinical asthmatic severity of children was found to exert no influence on TSLPR level. Conclusion: Thymic stromal lymphopoietin receptor might be a significant disease biomarker for asthma. The levels of TSLPR were found to be higher in asthmatic patients than in healthy children, but were found to be not different between allergic and nonallergic asthmatic patients. ? 2016 Stichting European Society for Clinical Investigation Journal Foundation. Published by John Wiley & Sons Ltd. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84964733910&doi=10.1111%2feci.12623&partnerID=40&md5=938d0f46e2d50d05b7f3aab2007cf140 https://scholars.lib.ntu.edu.tw/handle/123456789/567778 |
ISSN: | 0014-2972 | DOI: | 10.1111/eci.12623 | SDG/關鍵字: | biological marker; cytokine receptor; immunoglobulin E; interleukin 7 receptor alpha; messenger RNA; thymic stromal lymphopoietin; thymic stromal lymphopoietin receptor; unclassified drug; CRLF2 protein, human; cytokine; cytokine receptor; immunoglobulin E; interleukin 7 receptor; messenger RNA; thymic stromal lymphopoietin; allergic asthma; Article; asthma symptom score; blood sampling; child; controlled study; Dermatophagoides; disease severity; female; gene expression; human; immunoglobulin blood level; immunomodulation; major clinical study; male; priority journal; protein blood level; protein expression; protein secretion; real time polymerase chain reaction; respiratory tract disease assessment; reverse transcription polymerase chain reaction; upregulation; animal; asthma; case control study; genetics; immunology; metabolism; mite; severity of illness index; upregulation; Animals; Asthma; Case-Control Studies; Child; Cytokines; Female; Humans; Immunoglobulin E; Male; Mites; Real-Time Polymerase Chain Reaction; Receptors, Cytokine; Receptors, Interleukin-7; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Severity of Illness Index; Up-Regulation |
顯示於: | 醫學系 |
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