https://scholars.lib.ntu.edu.tw/handle/123456789/567824
標題: | Association of NLRP3 and CARD8 genetic polymorphisms with juvenile idiopathic arthritis in a Taiwanese population | 作者: | Yang C.-A. Huang S.-T. BOR-LUEN CHIANG |
公開日期: | 2014 | 出版社: | Informa Healthcare | 卷: | 43 | 期: | 2 | 起(迄)頁: | 146-152 | 來源出版物: | Scandinavian Journal of Rheumatology | 摘要: | Objectives: An elevated interleukin (IL)-1β response in peripheral blood mononuclear cells (PBMCs) has been observed in systemic juvenile idiopathic arthritis (sJIA), suggesting a role for inflammasomes in the pathogenesis of JIA. We aimed to determine whether genetic polymorphisms of the NLRP3 inflammasome components confer risk for oligoarticular and polyarticular JIA in a Taiwanese population. Method: A total of 118 JIA patients and 103 healthy controls were genotyped for rs4353135 OR2B11/NLRP3 and rs2043211 CARD8 polymorphisms. Clinical laboratory data and serum IL-1β of JIA patients were evaluated by medical chart review and enzyme-linked immunosorbent assay (ELISA), respectively. The production of IL-17 in lymphocytes of different genotype carriers was measured using flow cytometry. Results: The variant rs4353135 G allele carrier conferred increased risk for oligoarticular and polyarticular JIA. The G allele was also found to be associated with higher levels of clinical inflammatory markers. Moreover, G variant carriers enhanced the lymphocyte IL-17 response. The G/G genotype further increased the need for treatment with the tumour necrosis factor (TNF) inhibitor etanercept. Conclusions: Our data indicate that the rs4353135 OR2B11/NLRP3 polymorphism might be functional in, and could contribute to, the pathophysiology of oligoarticular and polyarticular JIA in a Taiwanese population. ? 2014 Taylor & Francis on license from Scandinavian Rheumatology Research Foundation. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84895760843&doi=10.3109%2f03009742.2013.834962&partnerID=40&md5=5fb9e817aeaa401043f745d80372d300 https://scholars.lib.ntu.edu.tw/handle/123456789/567824 |
ISSN: | 0300-9742 | DOI: | 10.3109/03009742.2013.834962 | SDG/關鍵字: | adaptor protein; caspase recruitment domain protein 8; cryopyrin; DNA; inflammasome; interleukin 17; interleukin 1beta; unclassified drug; adolescent; article; case control study; child; Chinese; chronic disease; controlled study; cytokine production; enzyme linked immunosorbent assay; ethnic group; female; flow cytometry; genetic polymorphism; genotype; human; immune system; juvenile rheumatoid arthritis; major clinical study; male; onset age; pathophysiology; peripheral blood mononuclear cell; polymerase chain reaction; priority journal; single nucleotide polymorphism; Taiwan; Adolescent; Alleles; Arthritis, Juvenile; CARD Signaling Adaptor Proteins; Carrier Proteins; Case-Control Studies; Child; Child, Preschool; Female; Genetic Predisposition to Disease; Genotype; Homozygote; Humans; Immunoglobulin G; Inflammasomes; Interleukin-17; Interleukin-1beta; Male; Neoplasm Proteins; Polymorphism, Single Nucleotide; Receptors, Tumor Necrosis Factor; Taiwan; Tumor Necrosis Factor-alpha |
顯示於: | 醫學系 |
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