https://scholars.lib.ntu.edu.tw/handle/123456789/567983
標題: | Inhibition of experimental autoimmune anterior uveitis by adenovirus-mediated transfer of the interleukin-10 gene | 作者: | Fang I.-M. CHANG-PING LIN CHANG-HAO YANG BOR-LUEN CHIANG CHUNG-MAY YANG Chau L.-Y. MUH-SHY CHEN |
公開日期: | 2005 | 卷: | 21 | 期: | 6 | 起(迄)頁: | 420-428 | 來源出版物: | Journal of Ocular Pharmacology and Therapeutics | 摘要: | Aims: The aim of this study was to investigate the efficiency of adenoviral-mediated transfer of the interleukin (IL)-10 gene for inhibition of experimental autoimmune anterior uveitis, a rat model of human acute anterior uveitis. Uveitis was induced in the Lewis rat by simultaneous injection of melanin-associated antigen intraperitoneally (i.p.) and into the left footpad. The animals were treated by systemic administration of adenoviral construct expressing IL-10 (Ad-IL-10) or Ad-Mock carrying no cytokine transgene. Results: A significant reduction in ocular inflammation was noted for rats that received one or two divided i.p. administrations of Ad-IL-10 (one 10 × 10 9 and two 5 × 109 particles of adenoviral construct, respectively), as judged by reduced clinical scores and decreased leukocyte infiltration in the anterior chamber and confirmed by histological examinations, relative to control animals. Systemic Ad-IL-10, treatment also revealed a higher serum level of IL-10, compared to the controls. Conclusions: The results suggest that systemic adenovirus-mediated IL-10 gene therapy has an anti-inflammatory effect on immune-mediated ocular inflammation and that this approach may be promising for the treatment of acute anterior uveitis. ? Mary Ann Liebert, Inc. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-30544453442&doi=10.1089%2fjop.2005.21.420&partnerID=40&md5=5ee6f9eaba5767830d7b5e635d6eb065 https://scholars.lib.ntu.edu.tw/handle/123456789/567983 |
ISSN: | 1080-7683 | DOI: | 10.1089/jop.2005.21.420 | SDG/關鍵字: | interleukin 10; melanin; Adenovirus; animal experiment; animal model; anterior eye chamber; antiinflammatory activity; article; autoimmune disease; cell infiltration; controlled study; gene transfer; histology; human; human cell; iridocyclitis; leukocyte; nonhuman; rat; transgene; viral gene therapy; Adenoviridae; Animals; Anterior Chamber; Aqueous Humor; Autoimmune Diseases; Chemotaxis, Leukocyte; Disease Models, Animal; Gene Therapy; Gene Transfer Techniques; Genetic Vectors; Interleukin-10; Leukocyte Count; Lymph Nodes; Polymerase Chain Reaction; Rats; Rats, Inbred Lew; Tumor Necrosis Factor-alpha; Uveitis, Anterior |
顯示於: | 醫學系 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。