https://scholars.lib.ntu.edu.tw/handle/123456789/568035
標題: | Roles of keratinocyte inflammation in oral cancer: Regulating the prostaglandin E2, interleukin-6 and TNF-α production of oral epithelial cells by areca nut extract and arecoline | 作者: | JIIANG-HUEI JENG Wang Y.-J. BOR-LUEN CHIANG Lee P.-H. Chan C.-P. Ho Y.-S. TONG-MEI WANG JANG-JAER LEE Hahn L.-J. Chang M.-C. |
公開日期: | 2003 | 出版社: | Oxford University Press | 卷: | 24 | 期: | 8 | 起(迄)頁: | 1301-1315 | 來源出版物: | Carcinogenesis | 摘要: | Betel quid (BQ) chewing is an etiologic factor of oral cancer and submucus fibrosis (OSF). Keratinocyte inflammation is crucial for the pathogenesis of cancer and tissue fibrosis. We found that areca nut (AN) extract (100-400 μg/ml) induced PGE2 production by KB cells by 2.34- to 23.1-fold and also TNF-α production by gingival keratinocytes (GK). Arecoline (0.2-1.2 mM) elevated PGE2 production by KB cells by 2.5- to 6.1-fold. AN extract (200-400 μg/ml also induced IL-6 production by GK (7.5- to 8.4-fold) and KB cells. In contrast, arecoline (0.1-1.2 mM) suppressed IL-6 production by GK and KB cells, with 42-81 and 41-63% inhibition, respectively. A 48 h exposure of GK to 800-1200 μg/ml AN extract led to 37-69% cell death. Arecoline cytotoxicity to GK was noted at concentrations of 0.8-1.2 mM, which led to 28-38% cell death. AN extract (400-800 μg/ml) induced Cox-2 and IL-6 mRNA expression and also COX-2 protein production by KB cells. IL-6 (5-100 ng/ml) suppressed GK growth by 20-33%, but enhanced oral fibroblast (OMF) and KB cell growth. PGE2 (0.05-5 μg/ml) and anti-IL-6 antibody (ab) (50-1000 ng/ml) showed little effect on GK and KB cell growth. Incubation of GK and KB cells with aspirin, antiIL-6 ab and anti-TNF-α ab showed little effect on arecoline- and AN-induced cytotoxicity, cell cycle arrest and apoptosis. Exposure to anti-TNF-α ab slightly affected arecoline- and AN-modulated PGE2 and IL-6 production by GK and KB cells. Arecoline- and AN-conditioned medium decreased phytohemagglutinin-mediated CD4+ and CD8+ T cell activation. These results indicate that BQ chewing contributes to the pathogenesis of cancer and OSF by impairing T cell activation and by induction of PGE2, TNF-α and IL-6 production, which affect oral mucosal inflammation and growth of OMF and oral epithelial cells. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-0042889276&doi=10.1093%2fcarcin%2fbgg083&partnerID=40&md5=9d33088f4e21e5c4b65cc0a2761255a3 https://scholars.lib.ntu.edu.tw/handle/123456789/568035 |
ISSN: | 0143-3334 | DOI: | 10.1093/carcin/bgg083 | SDG/關鍵字: | acetylsalicylic acid; arecoline; betel extract; CD4 antigen; CD8 antigen; cyclooxygenase 2; interleukin 6; interleukin 6 antibody; messenger RNA; phytohemagglutinin; prostaglandin E2; tumor necrosis factor alpha; tumor necrosis factor alpha antibody; apoptosis; betel nut; carcinogenesis; cell death; concentration (parameters); controlled study; cytokine production; cytotoxicity; epithelium cell; fibroblast; gene expression; human; human cell; keratinocyte; mouth cancer; mouth mucosa; priority journal; regulatory mechanism; review; T lymphocyte activation |
顯示於: | 醫學系 |
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