https://scholars.lib.ntu.edu.tw/handle/123456789/570284
標題: | Targeted Drug Delivery Systems Mediated by a Novel Peptide in Breast Cancer Therapy and Imaging | 作者: | Lu R.-M. Chen M.-S. Chang D.-K. Chiu C.-Y. Lin W.-C. Yan S.-L. YI-PING WANG Kuo Y.-S. Yeh C.-Y. Lo A. Wu H.-C. |
公開日期: | 2013 | 卷: | 8 | 期: | 6 | 起(迄)頁: | e66128 | 來源出版物: | PLoS ONE | 摘要: | Targeted delivery of drugs to tumors represents a significant advance in cancer diagnosis and therapy. Therefore, development of novel tumor-specific ligands or pharmaceutical nanocarriers is highly desirable. In this study, we utilized phage display to identify a new targeting peptide, SP90, which specifically binds to breast cancer cells, and recognizes tumor tissues from breast cancer patients. We used confocal and electron microscopy to reveal that conjugation of SP90 with liposomes enables efficient delivery of drugs into cancer cells through endocytosis. Furthermore, in vivo fluorescent imaging demonstrated that SP90-conjugated quantum dots possess tumor-targeting properties. In tumor xenograft and orthotopic models, SP90-conjugated liposomal doxorubicin was found to improve the therapeutic index of the chemotherapeutic drug by selectively increasing its accumulation in tumors. We conclude that the targeting peptide SP90 has significant potential in improving the clinical benefits of chemotherapy in the treatment and the diagnosis of breast cancer. ? 2013 Lu et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878929333&doi=10.1371%2fjournal.pone.0066128&partnerID=40&md5=156c5181c81a1afc9599069a264a8bef https://scholars.lib.ntu.edu.tw/handle/123456789/570284 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0066128 | SDG/關鍵字: | antineoplastic agent; doxorubicin; doxorubicin serylmethionylaspartylprolylphenylalanylleucylphenylalanylglutaminylleucylleucylglutaminylleucine conjugate; doxorubicin threonylaspartylserylisoleucylleucylarginylseryltyrosylaspartylglycylglycylglycine conjugate; liposome; quantum dot; serylmethionylaspartylprolylphenylalanylleucylphenylalanylglutaminylleucylleucylglutaminylleucine; sulforhodamine B; threonylaspartylserylisoleucylleucylarginylseryltyrosylaspartylglycylglycylglycine; unclassified drug; animal experiment; animal model; article; binding affinity; breast cancer; cancer cell; cancer chemotherapy; cancer diagnosis; cancer tissue; cell interaction; controlled study; diagnostic imaging; drug conjugation; drug cytotoxicity; drug delivery system; drug efficacy; early diagnosis; endocytosis; female; fluorescence imaging; human; human cell; human tissue; in vivo study; molecularly targeted therapy; multiple cycle treatment; nonhuman; nucleotide sequence; phage display; protein analysis; protein binding; protein purification; protein synthesis; protein targeting; treatment duration; treatment outcome; tumor vascularization; tumor volume; tumor xenograft; Animals; Breast Neoplasms; Cell Line, Tumor; Cell Proliferation; Diagnostic Imaging; Doxorubicin; Drug Delivery Systems; Female; Humans; Mice; Mice, SCID; Peptides; Polyethylene Glycols |
顯示於: | 臨床牙醫學研究所 |
在 IR 系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。