https://scholars.lib.ntu.edu.tw/handle/123456789/570284
Title: | Targeted Drug Delivery Systems Mediated by a Novel Peptide in Breast Cancer Therapy and Imaging | Authors: | Lu R.-M. Chen M.-S. Chang D.-K. Chiu C.-Y. Lin W.-C. Yan S.-L. YI-PING WANG Kuo Y.-S. Yeh C.-Y. Lo A. Wu H.-C. |
Issue Date: | 2013 | Journal Volume: | 8 | Journal Issue: | 6 | Start page/Pages: | e66128 | Source: | PLoS ONE | Abstract: | Targeted delivery of drugs to tumors represents a significant advance in cancer diagnosis and therapy. Therefore, development of novel tumor-specific ligands or pharmaceutical nanocarriers is highly desirable. In this study, we utilized phage display to identify a new targeting peptide, SP90, which specifically binds to breast cancer cells, and recognizes tumor tissues from breast cancer patients. We used confocal and electron microscopy to reveal that conjugation of SP90 with liposomes enables efficient delivery of drugs into cancer cells through endocytosis. Furthermore, in vivo fluorescent imaging demonstrated that SP90-conjugated quantum dots possess tumor-targeting properties. In tumor xenograft and orthotopic models, SP90-conjugated liposomal doxorubicin was found to improve the therapeutic index of the chemotherapeutic drug by selectively increasing its accumulation in tumors. We conclude that the targeting peptide SP90 has significant potential in improving the clinical benefits of chemotherapy in the treatment and the diagnosis of breast cancer. ? 2013 Lu et al. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84878929333&doi=10.1371%2fjournal.pone.0066128&partnerID=40&md5=156c5181c81a1afc9599069a264a8bef https://scholars.lib.ntu.edu.tw/handle/123456789/570284 |
ISSN: | 1932-6203 | DOI: | 10.1371/journal.pone.0066128 | SDG/Keyword: | antineoplastic agent; doxorubicin; doxorubicin serylmethionylaspartylprolylphenylalanylleucylphenylalanylglutaminylleucylleucylglutaminylleucine conjugate; doxorubicin threonylaspartylserylisoleucylleucylarginylseryltyrosylaspartylglycylglycylglycine conjugate; liposome; quantum dot; serylmethionylaspartylprolylphenylalanylleucylphenylalanylglutaminylleucylleucylglutaminylleucine; sulforhodamine B; threonylaspartylserylisoleucylleucylarginylseryltyrosylaspartylglycylglycylglycine; unclassified drug; animal experiment; animal model; article; binding affinity; breast cancer; cancer cell; cancer chemotherapy; cancer diagnosis; cancer tissue; cell interaction; controlled study; diagnostic imaging; drug conjugation; drug cytotoxicity; drug delivery system; drug efficacy; early diagnosis; endocytosis; female; fluorescence imaging; human; human cell; human tissue; in vivo study; molecularly targeted therapy; multiple cycle treatment; nonhuman; nucleotide sequence; phage display; protein analysis; protein binding; protein purification; protein synthesis; protein targeting; treatment duration; treatment outcome; tumor vascularization; tumor volume; tumor xenograft; Animals; Breast Neoplasms; Cell Line, Tumor; Cell Proliferation; Diagnostic Imaging; Doxorubicin; Drug Delivery Systems; Female; Humans; Mice; Mice, SCID; Peptides; Polyethylene Glycols |
Appears in Collections: | 臨床牙醫學研究所 |
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