https://scholars.lib.ntu.edu.tw/handle/123456789/575360
標題: | Dielectrophoresis-based cellular microarray chip for anticancer drug screening in perfusion microenvironments | 作者: | Hsiung, L.-C. Chiang, C.-L. Wang, C.-H. Huang, Y.-H. Kuo, C.-T. Cheng, J.-Y. CHING-HUNG LIN Wu, V. Chou, H.-Y. Jong, D.-S. Lee, H. ANDREW WO |
公開日期: | 2011 | 出版社: | Royal Society of Chemistry | 卷: | 11 | 期: | 14 | 起(迄)頁: | 2333-2342 | 來源出版物: | Lab on a Chip | 摘要: | We present a dielectrophoresis (DEP)-based cellular microarray chip for cell-based anticancer drug screening in perfusion microenvironments. Human breast cancer cells, MCF7, were seeded into the chip and patterned via DEP forces onto the planar interdigitated ring electrode (PIRE) arrays. Roughly, only one third of the cell amount was required for the chip compared to that for a 96-well plate control. Drug concentrations (cisplatin or docetaxel) were stably generated by functional integration of a concentration gradient generator (CGG) and an anti-crosstalk valve (ACV) to treat cells for 24 hours. Cell viability was quantified using a dual staining method. Results of cell patterning show substantial uniformity of patterned cells (92 ± 5 cells per PIRE). Furthermore, after 24 hour drug perfusion, no statistical significance in dose-responses between the chip and the 96-well plate controls was found. The IC50 value from the chip also concurred with the values from the literature. Moreover, the perfusion culture exhibited reproducibility of drug responses of cells on different PIREs in the same chamber. The chip would enable applications where cells are of limited supply, and supplement microfluidic perfusion cultures for clinical practices. ? 2011 The Royal Society of Chemistry. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-80051771450&doi=10.1039%2fc1lc20147f&partnerID=40&md5=843c06414c58d6771be5c3181db6ff44 https://scholars.lib.ntu.edu.tw/handle/123456789/575360 |
ISSN: | 1473-0197 | DOI: | 10.1039/c1lc20147f | SDG/關鍵字: | antineoplastic agent; cisplatin; docetaxel; article; biochip; breast cancer; cancer cell; controlled study; drug screening; electrophoresis; human; human cell; IC 50; microarray analysis; microchip analysis; microfluidic analysis; priority journal; tumor microenvironment |
顯示於: | 醫學系 |
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