Dielectrophoresis-based cellular microarray chip for anticancer drug screening in perfusion microenvironments
Journal
Lab on a Chip
Journal Volume
11
Journal Issue
14
Pages
2333-2342
Date Issued
2011
Author(s)
Hsiung, L.-C.
Chiang, C.-L.
Wang, C.-H.
Huang, Y.-H.
Kuo, C.-T.
Cheng, J.-Y.
Wu, V.
Chou, H.-Y.
Jong, D.-S.
Lee, H.
Abstract
We present a dielectrophoresis (DEP)-based cellular microarray chip for cell-based anticancer drug screening in perfusion microenvironments. Human breast cancer cells, MCF7, were seeded into the chip and patterned via DEP forces onto the planar interdigitated ring electrode (PIRE) arrays. Roughly, only one third of the cell amount was required for the chip compared to that for a 96-well plate control. Drug concentrations (cisplatin or docetaxel) were stably generated by functional integration of a concentration gradient generator (CGG) and an anti-crosstalk valve (ACV) to treat cells for 24 hours. Cell viability was quantified using a dual staining method. Results of cell patterning show substantial uniformity of patterned cells (92 ± 5 cells per PIRE). Furthermore, after 24 hour drug perfusion, no statistical significance in dose-responses between the chip and the 96-well plate controls was found. The IC50 value from the chip also concurred with the values from the literature. Moreover, the perfusion culture exhibited reproducibility of drug responses of cells on different PIREs in the same chamber. The chip would enable applications where cells are of limited supply, and supplement microfluidic perfusion cultures for clinical practices. ? 2011 The Royal Society of Chemistry.
SDGs
Other Subjects
antineoplastic agent; cisplatin; docetaxel; article; biochip; breast cancer; cancer cell; controlled study; drug screening; electrophoresis; human; human cell; IC 50; microarray analysis; microchip analysis; microfluidic analysis; priority journal; tumor microenvironment
Publisher
Royal Society of Chemistry
Type
journal article