https://scholars.lib.ntu.edu.tw/handle/123456789/575768
標題: | Carbonized nanogels for simultaneous antibacterial and antioxidant treatment of bacterial keratitis | 作者: | Lin H.-Y Wang S.-W Mao J.-Y HUAN-TSUNG CHANG Harroun S.G Lin H.-J Huang C.-C Lai J.-Y. |
關鍵字: | Amino acids; Biocompatibility; Disintegration; Escherichia coli; Medical nanotechnology; Nanostructured materials; Salmonella; Staphylococcus aureus; Antioxidant properties; Antioxidant treatment; Corneal biocompatibility; Methicillin-resistant staphylococcus aureus; Multidrug resistants; Pseudomonas aeruginosa; Salmonella enteritidis; Specific interaction; Antioxidants | 公開日期: | 2021 | 卷: | 411 | 來源出版物: | Chemical Engineering Journal | 摘要: | To prevent visual loss, clinical therapy of severe bacterial keratitis (BK) demands simultaneous remedying of the damage caused by bacterial infection and its induced oxidative stress/inflammation of the cornea. Here, we have developed a one-step method to synthesize carbonized nanogels (CNGs) from biogenic quercetin (Qu) and lysine (Lys) as a bifunctional agent with antibacterial and antioxidant properties for topical BK therapy. These Qu/Lys-CNGs synthesized by dry heating inherit and amplify the advantages of two natural products, including excellent antioxidant capacity and high positive charge. The Qu/Lys-CNGs display broad-spectrum bacteriostatic effects against non-multidrug-resistant bacteria, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella enteritidis, and also against multidrug-resistant bacteria, methicillin-resistant Staphylococcus aureus. Disintegration of peripheral structures of the bacteria caused by Qu/Lys-CNGs is due to their strong and specific interaction with bacterial membranes. In vitro cytotoxicity and hemolysis assays and in vivo corneal biocompatibility evaluations revealed good biocompatibility of Qu/Lys-CNGs as a safe nanomedicine in ophthalmic drop formulation. Furthermore, the Qu/Lys-CNGs can penetrate into the cornea via epithelial tight junction opening, thereby exerting superior antibacterial and antioxidant therapeutic effects against S. aureus-induced keratitis in rabbits. Our results indicate Qu/Lys-CNGs could be an effective bifunctional agent for clinical applications to treat bacterial ocular diseases. ? 2021 Elsevier B.V. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85099612517&doi=10.1016%2fj.cej.2021.128469&partnerID=40&md5=26604bb348183ff321f2b4eeada0d02c https://scholars.lib.ntu.edu.tw/handle/123456789/575768 |
ISSN: | 13858947 | DOI: | 10.1016/j.cej.2021.128469 |
顯示於: | 化學系 |
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