https://scholars.lib.ntu.edu.tw/handle/123456789/582713
Title: | E-cadherin Interacts With Posttranslationally-Modified AGO2 to Enhance miRISC Activity | Authors: | Li, Jie-Ning Sun, Hui-Lung MING-YANG WANG Chen, Pai-Sheng |
Keywords: | AGO2; E-cadherin; miRISC activity; miRNA; protein stability | Issue Date: | 2021 | Journal Volume: | 9 | Source: | Frontiers in cell and developmental biology | Abstract: | MicroRNAs (miRNAs) are small non-coding RNAs which post-transcriptionally suppress target mRNAs expression and/or translation to modulate pathophyological processes. Expression and function of miRNAs are fine-tuned by a conserved biogenesis machinery involves two RNase-dependent processing steps of miRNA maturation and the final step of miRNA-induced silencing complex (miRISC)-mediated target silencing. A functional miRISC requires Argonaute 2 (AGO2) as an essential catalytic component which plays central roles in miRISC function. We uncovered a post-translational regulatory mechanism of AGO2 by E-cadherin. Mechanistically, E-cadherin activates ERK to phosphorylate AGO2, along with enhanced protein glycosylation. Consequently, the phosphorylated AGO2 was stabilized and ultimately resulted in induced miRISC activity on gene silencing. This study revealed a novel pathway for miRNA regulation through an E-cadherin-mediated miRISC activation. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/582713 | ISSN: | 2296-634X | DOI: | 10.3389/fcell.2021.671244 |
Appears in Collections: | 醫學院附設癌醫中心醫院(臺大癌醫) |
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