https://scholars.lib.ntu.edu.tw/handle/123456789/589319
標題: | Species identification of medically important fungi by use of real-time LightCycler PCR | 作者: | Hsu M.-C. Chen K.-W. Lo H.-J. YEE-CHUN CHEN Liao M.-H. Lin Y.-H. Li S.-Y. |
公開日期: | 2003 | 卷: | 52 | 期: | 12 | 起(迄)頁: | 1071-1076 | 來源出版物: | Journal of Medical Microbiology | 摘要: | Invasive fungal infection has become a major cause of morbidity and mortality in immunocompromised patients. Rapid identification of pathogenic fungi to species level is critical for disease treatment. A real-time LightCycler assay aiming at rapid detection and species identification of pathogenic fungi from clinical isolates was developed. Template DNAs of different species were amplified and detected in real time by employing SYBR Green fluorescent dye. The target sequences for species-level detection were located between the 18S and 28S rDNA. Seven fungal species encountered frequently in the clinical setting, Candida albicans, Candida glabrata, Candida krusei, Candida parapsilosis, Candida tropicalis, Candida guilliermondii and Cryptococcus neoformans, could be discriminated by species-specific primers and confirmed by melting-curve analyses. The range of linearity was from 1 ng to 1 pg (μl-1 water) and the sensitivity was 1 pg fungal DNA μl -1. Identification by this real-time PCR method matched biochemical identification for all 58 clinical strains. Therefore, the method is simple, rapid and sensitive enough for detection and identification of several fungal species. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542750263&doi=10.1099%2fjmm.0.05302-0&partnerID=40&md5=c3aaf2a2efc4bf82bddc1879d7e2fd50 https://scholars.lib.ntu.edu.tw/handle/123456789/589319 |
ISSN: | 0022-2615 | DOI: | 10.1099/jmm.0.05302-0 | SDG/關鍵字: | DNA; green fluorescent protein; ribosome DNA; water; analytic method; article; Candida albicans; Candida glabrata; Candida guilliermondii; Candida krusei; Candida tropicalis; controlled study; Cryptococcus neoformans; DNA determination; DNA template; fungal detection; fungal strain; fungus; fungus isolation; gene amplification; gene sequence; gene targeting; nonhuman; nucleotide sequence; priority journal; real time polymerase chain reaction; sequence alignment; species identification; human; isolation and purification; methodology; polymerase chain reaction; sensitivity and specificity; Fungi; Humans; Polymerase Chain Reaction; Sensitivity and Specificity |
顯示於: | 醫學系 |
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