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  4. Species identification of medically important fungi by use of real-time LightCycler PCR
 
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Species identification of medically important fungi by use of real-time LightCycler PCR

Journal
Journal of Medical Microbiology
Journal Volume
52
Journal Issue
12
Pages
1071-1076
Date Issued
2003
Author(s)
Hsu M.-C.
Chen K.-W.
Lo H.-J.
YEE-CHUN CHEN  
Liao M.-H.
Lin Y.-H.
Li S.-Y.
DOI
10.1099/jmm.0.05302-0
URI
https://www.scopus.com/inward/record.uri?eid=2-s2.0-1542750263&doi=10.1099%2fjmm.0.05302-0&partnerID=40&md5=c3aaf2a2efc4bf82bddc1879d7e2fd50
https://scholars.lib.ntu.edu.tw/handle/123456789/589319
Abstract
Invasive fungal infection has become a major cause of morbidity and mortality in immunocompromised patients. Rapid identification of pathogenic fungi to species level is critical for disease treatment. A real-time LightCycler assay aiming at rapid detection and species identification of pathogenic fungi from clinical isolates was developed. Template DNAs of different species were amplified and detected in real time by employing SYBR Green fluorescent dye. The target sequences for species-level detection were located between the 18S and 28S rDNA. Seven fungal species encountered frequently in the clinical setting, Candida albicans, Candida glabrata, Candida krusei, Candida parapsilosis, Candida tropicalis, Candida guilliermondii and Cryptococcus neoformans, could be discriminated by species-specific primers and confirmed by melting-curve analyses. The range of linearity was from 1 ng to 1 pg (μl-1 water) and the sensitivity was 1 pg fungal DNA μl -1. Identification by this real-time PCR method matched biochemical identification for all 58 clinical strains. Therefore, the method is simple, rapid and sensitive enough for detection and identification of several fungal species.
SDGs

[SDGs]SDG3

Other Subjects
DNA; green fluorescent protein; ribosome DNA; water; analytic method; article; Candida albicans; Candida glabrata; Candida guilliermondii; Candida krusei; Candida tropicalis; controlled study; Cryptococcus neoformans; DNA determination; DNA template; fungal detection; fungal strain; fungus; fungus isolation; gene amplification; gene sequence; gene targeting; nonhuman; nucleotide sequence; priority journal; real time polymerase chain reaction; sequence alignment; species identification; human; isolation and purification; methodology; polymerase chain reaction; sensitivity and specificity; Fungi; Humans; Polymerase Chain Reaction; Sensitivity and Specificity
Type
journal article

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