https://scholars.lib.ntu.edu.tw/handle/123456789/597751
標題: | Study effects of hypoxia-inducible factor (HIF) inhibitor on endothelial cell migration under oxygen gradients using microfluidic wound healing assay | 作者: | Shih, Hsiu Chen Lee, Tse Ang HSIAO-MEI WU Ko, Ping Liang Tung, Yi Chung |
關鍵字: | Endothelial cells (ECs) | Human umbilical vain endothelial cells (HUVECs) | Oxygen gradients | YC-1 | 公開日期: | 1-一月-2018 | 卷: | 3 | 來源出版物: | 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 | 摘要: | This paper studies the effects of hypoxia-inducible factor (HIF) inhibitor on endothelial cell migration under oxygen gradients. The experiments are conducted using microfluidic cell culture devices capable of generating oxygen gradients. The oxygen gradients are generated using a spatially confined chemical reaction method to eliminate direct chemical addition into cell culture medium, and the device is compatible with conventional cell incubators. The results indicate that the HIF inhibitor has minimal effects on endothelial polarized cell migration toward low oxygen side. The developed platform provides a powerful tool to study the endothelial cell behaviors under various oxygen conditions and drugs. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/597751 | ISBN: | 9781510897571 |
顯示於: | 生物機電工程學系 |
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