Study effects of hypoxia-inducible factor (HIF) inhibitor on endothelial cell migration under oxygen gradients using microfluidic wound healing assay
Journal
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
Journal Volume
3
ISBN
9781510897571
Date Issued
2018-01-01
Author(s)
Abstract
This paper studies the effects of hypoxia-inducible factor (HIF) inhibitor on endothelial cell migration under oxygen gradients. The experiments are conducted using microfluidic cell culture devices capable of generating oxygen gradients. The oxygen gradients are generated using a spatially confined chemical reaction method to eliminate direct chemical addition into cell culture medium, and the device is compatible with conventional cell incubators. The results indicate that the HIF inhibitor has minimal effects on endothelial polarized cell migration toward low oxygen side. The developed platform provides a powerful tool to study the endothelial cell behaviors under various oxygen conditions and drugs.
Subjects
Endothelial cells (ECs) | Human umbilical vain endothelial cells (HUVECs) | Oxygen gradients | YC-1
Type
conference paper