https://scholars.lib.ntu.edu.tw/handle/123456789/614726
Title: | Long-term feeding of high-fat plus high-fructose diet induces isolated impaired glucose tolerance and skeletal muscle insulin resistance in miniature pigs | Authors: | Hsu M.-C.; Wang M.-E.; Jiang Y.-F.; Liu H.-C.; Chen Y.-C.; Chiu C.-H. Wang, Mu-En YI-FAN JIANG Liu, Hung-Chang YI-CHEN CHEN MENG-CHIEH HSU |
Keywords: | fat; fructose; glucose; insulin; protein kinase B; adult; animal experiment; animal model; animal tissue; Article; body weight; carbohydrate diet; controlled study; dyslipidemia; enzyme phosphorylation; ex vivo study; fat intake; glucose blood level; hyperinsulinemia; impaired glucose tolerance; insulin blood level; insulin release; insulin resistance; insulin sensitivity; intravenous glucose tolerance test; lipid diet; liver tissue; low fat diet; male; minipig; muscle tissue; nonhuman; obesity; priority journal; skeletal muscle; soleus muscle; sugar intake | Issue Date: | 2017 | Publisher: | BioMed Central Ltd. | Journal Volume: | 9 | Journal Issue: | 1 | Source: | Diabetology and Metabolic Syndrome | Abstract: | Background: During the prediabetic development, the changes in β-cell function and tissue-specific insulin resistance have been described. However, there are conflicting views in insulin secretory capacity between early clinical observation and recent proposed mathematical model. On the basis of digestive and metabolic similarities with humans, swine have great potential as an animal model to investigate the progressive mechanisms of prediabetes. The aim of this study was to investigate the insulin secretory response and tissue-specific insulin resistance in a dietary-induced prediabetic porcine model. Methods: Adult male Taiwan Lee-Sung miniature pigs were randomized into two groups: (1) low-fat diet and (2) high-fat plus high-fructose diet (HFHF; 20.9% crude fat and 17.8% fructose). During the 12-month dietary intervention, body weights and blood glucose levels were measured monthly. Intravenous glucose tolerance test was used for measuring glucose tolerance and insulin secretory capacity. At the end of the experiment, liver and soleus muscle specimens were collected for ex vivo insulin sensitivity testing. Results: The results showed that the HFHF group had obesity, hyperinsulinemia, and dyslipidemia, but normal fasting glucose levels. The HFHF pigs exhibited enhanced first- and second-phase insulin secretion and high 2-h postload glucose levels in intravenous glucose tolerance test. Furthermore, the skeletal muscle specimens from the HFHF group were desensitized to insulin stimulation as shown by the lack of AKT Ser473 phosphorylation; however, the liver specimens remained a normal response. Conclusions: In conclusion, the HFHF diet-fed pigs developed isolated impaired glucose tolerance corresponding to prediabetes with an intense insulin secretory response and skeletal muscle insulin resistance. © 2017 The Author(s). |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85031319580&doi=10.1186%2fs13098-017-0281-6&partnerID=40&md5=ff182ce13513be3e4b67630591381ed4 https://scholars.lib.ntu.edu.tw/handle/123456789/614726 |
ISSN: | 17585996 | DOI: | 10.1186/s13098-017-0281-6 |
Appears in Collections: | 動物科學技術學系 |
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