https://scholars.lib.ntu.edu.tw/handle/123456789/634753
標題: | Integrative Analysis of Myofibroblast Transformation During Corneal Fibrosis | 作者: | Pai, Yun Ling Wu, Yueh Feng SUNG-JAN LIN |
關鍵字: | Corneal opacification | fibrosis | keratocyte | myofibroblast | scar | wound healing | 公開日期: | 10-十一月-2022 | 來源出版物: | ACM International Conference Proceeding Series | 摘要: | The transparent corneal stroma, which consists of regularly packed collagen fibrils and keratocytes, comprises 90% of the corneal thickness. Injury response in corneal stroma compromises corneal transparency due to overproduction of abnormal extracellular matrix by keratocyte-myofibroblast transformation. Corneal transplantation is considered the optimal solution to restore vision. Whether the keratocyte-myofibroblast transformation can be reversed remains unclear. In this study, we first developed a mouse corneal mechanical injury model. At 24h after the injury, α-SMA expression significantly increased, and SHG signals changed with respect to the collagen orientation. In addition, we isolated primary human keratocytes in advance; thereafter, we can decipher the keratocytes-myofibroblast transformation by adding TGF-β1 in the future. This proposal established three platforms, including a mouse injury model, primary human cell culture, and transgenic mice for targeting keratocytes. We will integrate these systems with single-cell transcriptomics, ATAC-sequencing, and proteomics to comprehensively explore keratocyte-myofibroblast transformation, and eventually, identify therapeutic targets for treating corneal fibrosis. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/634753 | ISBN: | 9781450397223 | DOI: | 10.1145/3574198.3574235 |
顯示於: | 醫學工程學研究所 |
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