https://scholars.lib.ntu.edu.tw/handle/123456789/637596
Title: | An Epidemiological Assessment of Cryptosporidium and Giardia spp. Infection in Pet Animals from Taiwan | Authors: | Hsu, Chia Hui Liang, Chi Chi, Shi Chien Lee, Kuan Ju CHUNG-HSI CHOU CHEN-SI LIN WEN-YUAN YANG |
Keywords: | assemblages | Cryptosporidium | Giardia duodenalis | glutamate dehydrogenase | molecular epidemiology | nested polymerase chain reaction | SSU-rRNA | zoonoses | β-giardin | Issue Date: | 1-Nov-2023 | Journal Volume: | 13 | Journal Issue: | 21 | Source: | Animals | Abstract: | Cryptosporidium spp. and Giardia duodenalis are enteric protozoan pathogens in humans. and animals. Companion animals infected with zoonotic species/assemblages are a matter of major public concern around the world. The objectives of the present study were to determine the prevalences of Cryptosporidium spp. and G. duodenalis infections and their co-infection statuses in dogs and cats living in Taiwan and to identify the species and assemblages. Fecal samples were collected from local animal shelters (n = 285) and a veterinary hospital (n = 108). Nested polymerase chain reaction (PCR) was performed using the SSU-rRNA, β-giardin, and glutamate dehydrogenase genes for Cryptosporidium spp. and G. duodenalis, respectively. Results showed that the overall prevalences of Cryptosporidium and G. duodenalis were 7.38% (29/393) and 10.69% (42/393). In addition, co-infection was detected in 1.02% (4/393) of all samples. Sample source, clinical sign, and breed may be risk factors that influence the infection rate. In Cryptosporidium-positive samples, C. canis and C. felis were detected most frequently. Although the canine-specific assemblages C and D (37/42) were dominant, the zoonotic human-specific assemblage A (1/42) was also found in Giardia-positive samples. Phylogenetic analysis revealed that most positive samples belonged to host-specific subtypes/assemblages, while some Cryptosporidium or Giardia-positive samples could be zoonotic. The findings suggested that pet animals could be a cause of zoonotic transmission, causing human cryptosporidiosis and giardiasis in Taiwan. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85176805891&doi=10.3390%2fani13213373&partnerID=40&md5=277976ba8248a917c4e7643ec5cc416b https://scholars.lib.ntu.edu.tw/handle/123456789/637596 |
ISSN: | 2076-2615 | DOI: | 10.3390/ani13213373 |
Appears in Collections: | 獸醫學系 |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.