https://scholars.lib.ntu.edu.tw/handle/123456789/638319
標題: | Elevated expression of thyroid hormone receptor alpha 2 (c-erb A- alpha 2) in nasopharyngeal carcinoma | 作者: | Lee, J W Chen, C L Juang, B T Chen, J Y Yang, C S SHIN-LIAN DOONG |
關鍵字: | C-erb A-α2 (TR-α2) | Differential display | Nasopharyngeal carcinoma | RNA in situ hybridization | 公開日期: | 十二月-2000 | 卷: | 83 | 期: | 12 | 來源出版物: | British journal of cancer | 摘要: | Differential display was used to identify genes differentially expressed between cultured normal nasal epithelial cells and nasopharyngeal carcinoma (NPC) cell lines. A 130 bp cDNA fragment showing homology with thyroid hormone receptor alpha2 (TR-alpha2 or c-erb A-alpha2) was identified in NPC cell lines. Northern blot analysis using the 130 bp cDNA fragment and a TR-alpha2 specific cDNA containing part of the coding region as probes, we were able to detect a 2.7 kb transcript corresponding to that of TR-alpha2 in NPC cell lines but not in normal nasal epithelial cells. RNA in situ hybridization was used to detect TR-alpha2 expression in clinical biopsies obtained from NPC patients and non-tumour controls. TR-alpha2 mRNA was detected in 1 out of 24 (4.2%) normal nasopharynx epithelium biopsies, in 5 out of 27 (18.5%) primary and 15 out of 24 (62.5%) recurrent tumours. The positive rate of TR-alpha2 expression in recurrent NPC biopsies was significantly higher than that in normal nasopharynx epithelium (P<0.00001). The relevance of the elevated expression of TR-alpha2 in the pathogenesis process of NPC was discussed. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/638319 | ISSN: | 0007-0920 | DOI: | 10.1054/bjoc.2000.1505 |
顯示於: | 微生物學科所 |
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