https://scholars.lib.ntu.edu.tw/handle/123456789/641607
Title: | Detection of Microorganisms in Body Fluids via MTT-PMS Assay | Authors: | Chen, Cheng-Han Tsao, Yu-Ting PO-TING YEH Liao, Yu-Hsiang Lee, Yi-Tzu Liao, Wan-Ting Wang, Yung-Chih Shen, Ching-Fen Cheng, Chao-Min |
Keywords: | MTT-PMS assay; bacterial detection; human body fluids; infectious diseases; point-of-care testing | Issue Date: | Jan-2022 | Journal Volume: | 12 | Journal Issue: | 1 | Start page/Pages: | 46 | Source: | Diagnostics (Basel, Switzerland) | Abstract: | Early detection of microorganisms is essential for the management of infectious diseases. However, this is challenging, as traditional culture methods are labor-intensive and time-consuming. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide-phenazine methosulfate (MTT-PMS) assay has been used to evaluate the metabolic activity in live cells and can thus be used for detecting living microorganisms. With the addition of NaOH and Tris-EDTA, the same approach can be accelerated (within 15 min) and used for the quick detection of common bacterial pathogens. The assay results can be evaluated colorimetrically or semi-quantitatively. Here, the quick detection by MTT-PMS assay was further investigated. The assay had a detection limit of approximately 104 CFU/mL. In clinical evaluations, we used the MTT-PMS assay to detect clinical samples and bacteriuria (>105 CFU/mL). The negative predictive value of the MTT-PMS assay for determining bacteriuria was 79.59% but was 100% when the interference of abnormal blood was excluded. Thus, the MTT-PMS assay might be a potential "rule-out" tool for bacterial detection in clinical samples, at a cost of approximately USD 1 per test. Owing to its low cost, rapid results, and easy-to-use characteristics, the MTT-PMS assay may be a potential tool for microorganism detection. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/641607 | ISSN: | 2075-4418 | DOI: | 10.3390/diagnostics12010046 |
Appears in Collections: | 醫學院附設醫院 (臺大醫院) |
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