Recording signal transduction dynamics with unprecedented temporal resolution
Journal
Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
ISBN
9780979806452
Date Issued
2012-01-01
Author(s)
Abstract
The binding of ligands to receptors initiates signal transduction. Our understanding of the early signal transduction events is limited by the poor temporal resolution of current analytical methods. To address this challenge we have developed a microfluidic strategy for capturing these processes with millisecond temporal resolution. A single cell continuous flow processing method based on deterministic lateral displacement principles was developed and used for cell triggering with microsecond precision. Using precisely defined ligand incubation periods the sub-second receptor tyrosine kinase autophosphorylation dynamics were investigated.
Subjects
Deterministic lateral displacement | IGF-1 receptor | Microfluidics | Signal transduction | Single cells
Type
conference paper