https://scholars.lib.ntu.edu.tw/handle/123456789/635630
標題: | Recording signal transduction dynamics with unprecedented temporal resolution | 作者: | YA-YU CHIANG Stewart, Joanna Gizewski, Carsten Ehrhard, Peter Janasek, Dirk West, Jonathan |
關鍵字: | Deterministic lateral displacement | IGF-1 receptor | Microfluidics | Signal transduction | Single cells | 公開日期: | 1-一月-2012 | 來源出版物: | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 | 摘要: | The binding of ligands to receptors initiates signal transduction. Our understanding of the early signal transduction events is limited by the poor temporal resolution of current analytical methods. To address this challenge we have developed a microfluidic strategy for capturing these processes with millisecond temporal resolution. A single cell continuous flow processing method based on deterministic lateral displacement principles was developed and used for cell triggering with microsecond precision. Using precisely defined ligand incubation periods the sub-second receptor tyrosine kinase autophosphorylation dynamics were investigated. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/635630 | ISBN: | 9780979806452 |
顯示於: | 機械工程學系 |
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