The synthesis of antigens and the production of antibodies against patulin derivatives
Journal
Journal of Food and Drug Analysis
Journal Volume
7
Journal Issue
1
Pages
65-72
Date Issued
1999
Author(s)
Abstract
Patulin hemisuccinate (P-HS) was synthesized and conjugated to bovine serum albumin (BSA) as the hapten-protein conjugation for immunization. Polyclonal antibodies were obtained from the antiascites of six immunized mice. Titers and specificity were determined using an indirect ELISA. The results indicated that three of the six mice were better immunized and that the antibodies acted strongly against patulin-hemisuccinate-BSA. Slight ELISA responses of the antibodies against patulin alone showed the potential for developing monoclonal antibodies.
Subjects
Patulin derivatives; Polyclonal antibody
Other Subjects
bovine serum albumin; hapten; hapten antibody; patulin; patulin hemisuccinate; polyclonal antibody; unclassified drug; animal experiment; antibody production; antibody specificity; antibody titer; antigen binding; article; ascites fluid; controlled study; drug conjugation; drug structure; drug synthesis; female; immunogenicity; intramuscular drug administration; intraperitoneal drug administration; mouse; nonhuman; reaction analysis; subcutaneous drug administration
Type
journal article