Simultaneous genotyping of single nucleotide polymorphisms in the IL-1 gene complex by multiplex polymerase chain reaction-restriction fragment length polymorphism
Journal
Journal of Immunological Methods
Journal Volume
267
Journal Issue
2
Pages
151-156
Date Issued
2002
Author(s)
Abstract
The interleukin-1 (IL-1) gene complex consists of the IL-1α, IL-1β and IL-1 receptor antagonist genes. Single-nucleotide polymorphisms (SNP) in all three genes have been associated with human diseases. In this study, primers containing mismatches at 1-3 nucleotide positions were designed to incorporate a restriction site for endonuclease AlwNI or XcmI in the presence of allele-specific nucleotides at the polymorphic positions. Based on this technique, a simple and robust multiplex polymerase chain reaction/restriction fragment length polymorphism (multiplex PCR/RFLP) assay was developed to determine simultaneously three to four informative SNPs (IL-1β/+3954, IL-1β/-511 and IL-1Ra/9261 or IL-1α/-889, IL-1β/-31, IL-1β/5810 and IL-1Ra/11100 SNPs) in the IL-1 gene complex. ? 2002 Elsevier Science B.V. All rights reserved.
SDGs
Other Subjects
endonuclease; interleukin 1 complex; interleukin 1 receptor blocking agent; interleukin 1alpha; interleukin 1beta; nucleotide; unclassified drug; allele; article; comparative study; genetic analysis; genotype; nucleotide sequence; polymerase chain reaction; priority journal; restriction fragment length polymorphism; restriction mapping; restriction site; single nucleotide polymorphism
Publisher
Elsevier
Type
journal article