https://scholars.lib.ntu.edu.tw/handle/123456789/568769
標題: | Simultaneous genotyping of single nucleotide polymorphisms in the IL-1 gene complex by multiplex polymerase chain reaction-restriction fragment length polymorphism | 作者: | LI-HUI TSENG PEI-JER CHEN Lin M.-T. Singleton K. Martin E.G. Yen A.-H. Martin P.J. Hansen J.A. |
公開日期: | 2002 | 出版社: | Elsevier | 卷: | 267 | 期: | 2 | 起(迄)頁: | 151-156 | 來源出版物: | Journal of Immunological Methods | 摘要: | The interleukin-1 (IL-1) gene complex consists of the IL-1α, IL-1β and IL-1 receptor antagonist genes. Single-nucleotide polymorphisms (SNP) in all three genes have been associated with human diseases. In this study, primers containing mismatches at 1-3 nucleotide positions were designed to incorporate a restriction site for endonuclease AlwNI or XcmI in the presence of allele-specific nucleotides at the polymorphic positions. Based on this technique, a simple and robust multiplex polymerase chain reaction/restriction fragment length polymorphism (multiplex PCR/RFLP) assay was developed to determine simultaneously three to four informative SNPs (IL-1β/+3954, IL-1β/-511 and IL-1Ra/9261 or IL-1α/-889, IL-1β/-31, IL-1β/5810 and IL-1Ra/11100 SNPs) in the IL-1 gene complex. ? 2002 Elsevier Science B.V. All rights reserved. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-84983723321&doi=10.1016%2fS0022-1759%2802%2900165-5&partnerID=40&md5=4917696f453b8f5d74a92495a7a436d1 https://scholars.lib.ntu.edu.tw/handle/123456789/568769 |
ISSN: | 0022-1759 | DOI: | 10.1016/S0022-1759(02)00165-5 | SDG/關鍵字: | endonuclease; interleukin 1 complex; interleukin 1 receptor blocking agent; interleukin 1alpha; interleukin 1beta; nucleotide; unclassified drug; allele; article; comparative study; genetic analysis; genotype; nucleotide sequence; polymerase chain reaction; priority journal; restriction fragment length polymorphism; restriction mapping; restriction site; single nucleotide polymorphism |
顯示於: | 臨床醫學研究所 |
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