Identification of targets of miR-200b by a SILAC-based quantitative proteomic approach
Journal
EuPA Open Proteomics
Journal Volume
4
Pages
10-17
Date Issued
2014
Author(s)
Marimuthu A.
Selvan L.D.N.
Renuse S.
Nirujogi R.S.
Kumar P.
Pinto S.M.
Rajagopalan S.
Pandey A.
Harsha H.C.
Chatterjee A.
Abstract
miRNAs regulate gene expression by binding to cognate mRNAs causing mRNA degradation or translational repression. Mass spectrometry-based proteomic analysis is being widely used to identify miRNA targets. The miR-200b miRNA cluster is often overexpressed in multiple cancer types, but the identity of the targets remains elusive. Using SILAC-based analysis, we examined the effects of overexpression of a miR-200b mimic or a control miRNA in fibrosarcoma cells. We identified around 300 potential targets of miR-200b based on a change in the expression of protein levels. We validated a subset of potential targets at the transcript level using quantitative PCR.
SDGs
Publisher
Elsevier
Type
journal article