A microfluidic microwell device operated by the automated microfluidic control system for surface-enhanced Raman scattering-based antimicrobial susceptibility testing
Journal
Biosensors & bioelectronics
Journal Volume
191
Date Issued
2021-11-01
Author(s)
Liao, Cheng-Chieh
Chen, Yi-Zih
Lin, Shang-Jyun
Cheng, Ho-Wen
Wang, Juen-Kai
Wang, Yuh-Lin
Abstract
Bloodstream infection (BSI) is a serious public health issue worldwide. Timely and effective antibiotics for controlling infection are crucial towards patient outcomes. However, the current culture-based methods of identifying bacteria and antimicrobial susceptibility testing (AST) remain labor-intensive and time-consuming, and are unable to provide early support to physicians in critical hours. To improve the effectiveness of early antibiotic therapy, Surface-enhanced Raman scattering (SERS) technology, has been used in bacterial detection and AST based on its high specificity and label-free features. To simplify sample preparation steps in SERS-AST, we proposed an automated microfluidic control system to integrate all required procedures into a single device. Our preliminary results demonstrated the system can achieve on-chip reagent replacement, bacteria trapping, and buffer exchange. Finally, in-situ SERS-AST was performed within 3.5 h by loading isolates of ampicilin susceptible and resistant E. coli and clear discrimination of two strains under antibiotic treatment was demonstrated. Overall, our system can standardize and simplify the SERS-AST protocol and implicate parallel bacterial detection. This prototypical integration demonstrates timely microbiological support to optimize early antibiotic therapy for fighting bacteremia.
Subjects
Antimicrobial susceptibility testing (AST); Automated microfluidic control system; Microwell; Surface-enhanced Raman scattering (SERS)
SDGs
Other Subjects
Antibiotics; Automation; Escherichia coli; Microchannels; Raman scattering; Surface scattering; Antibiotic therapy; Antimicrobial susceptibility testing; Automated microfluidic control system; Bacterial detection; Enhanced Raman scattering; Micro wells; M
Type
journal article