https://scholars.lib.ntu.edu.tw/handle/123456789/588139
標題: | A microfluidic microwell device operated by the automated microfluidic control system for surface-enhanced Raman scattering-based antimicrobial susceptibility testing | 作者: | Liao, Cheng-Chieh Chen, Yi-Zih Lin, Shang-Jyun Cheng, Ho-Wen Wang, Juen-Kai Wang, Yuh-Lin YIN-YI HAN NIEN-TSU HUANG |
關鍵字: | Antimicrobial susceptibility testing (AST); Automated microfluidic control system; Microwell; Surface-enhanced Raman scattering (SERS) | 公開日期: | 1-十一月-2021 | 卷: | 191 | 來源出版物: | Biosensors & bioelectronics | 摘要: | Bloodstream infection (BSI) is a serious public health issue worldwide. Timely and effective antibiotics for controlling infection are crucial towards patient outcomes. However, the current culture-based methods of identifying bacteria and antimicrobial susceptibility testing (AST) remain labor-intensive and time-consuming, and are unable to provide early support to physicians in critical hours. To improve the effectiveness of early antibiotic therapy, Surface-enhanced Raman scattering (SERS) technology, has been used in bacterial detection and AST based on its high specificity and label-free features. To simplify sample preparation steps in SERS-AST, we proposed an automated microfluidic control system to integrate all required procedures into a single device. Our preliminary results demonstrated the system can achieve on-chip reagent replacement, bacteria trapping, and buffer exchange. Finally, in-situ SERS-AST was performed within 3.5 h by loading isolates of ampicilin susceptible and resistant E. coli and clear discrimination of two strains under antibiotic treatment was demonstrated. Overall, our system can standardize and simplify the SERS-AST protocol and implicate parallel bacterial detection. This prototypical integration demonstrates timely microbiological support to optimize early antibiotic therapy for fighting bacteremia. |
URI: | https://scholars.lib.ntu.edu.tw/handle/123456789/588139 | ISSN: | 09565663 | DOI: | 10.1016/j.bios.2021.113483 | SDG/關鍵字: | Antibiotics; Automation; Escherichia coli; Microchannels; Raman scattering; Surface scattering; Antibiotic therapy; Antimicrobial susceptibility testing; Automated microfluidic control system; Bacterial detection; Enhanced Raman scattering; Micro wells; M |
顯示於: | 醫學系 |
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