Evaluation and comparison of the pathogenicity and host immune responses induced by a G2b Taiwan porcine epidemic diarrhea virus (strain pintung 52) and its highly cell-culture passaged strain in conventional 5-week-old pigs
Journal
Viruses
Journal Volume
9
Journal Issue
5
Date Issued
2017
Author(s)
Chang Y.-C
Kao C.-F
Chang C.-Y
Jeng C.-R
Tsai P.-S
Pang V.F
Chiou H.-Y
Peng J.-Y
Cheng I.-C
Abstract
A genogroup 2b (G2b) porcine epidemic diarrhea virus (PEDV) Taiwan Pintung 52 (PEDVPT) strain was isolated in 2014. The pathogenicity and host antibody responses elicited by low-passage (passage 5; PEDVPT-P5) and high-passage (passage 96; PEDVPT-P96) PEDVPT strains were compared in post-weaning PEDV-seronegative pigs by oral inoculation. PEDVPT-P5- inoculation induced typical diarrhea during 1-9 days post inoculation with fecal viral shedding persisting for 26 days. Compared to PEDVPT-P5, PEDVPT-P96 inoculation induced none-to-mild diarrhea and lower, delayed fecal viral shedding. Although PEDVPT-P96 elicited slightly lower neutralizing antibodies and PEDV-specific immunoglobulin G (IgG) and immunoglobulin A (IgA) titers, a reduction in pathogenicity and viral shedding of the subsequent challenge with PEDVPT-P5 were noted in both PEDVPT-P5- and PEDVPT-P96-inoculated pigs. Alignment and comparison of full-length sequences of PEDVPT-P5 and PEDVPT-P96 revealed 23 nucleotide changes and resultant 19 amino acid substitutions in non-structure proteins 2, 3, 4, 9, 14, 15, spike, open reading frame 3 (ORF3), and membrane proteins with no detectable deletion or insertion. The present study confirmed the pathogenicity of the PEDVPT isolate in conventional post-weaning pigs. Moreover, data regarding viral attenuation and potency of induced antibodies against PEDVPT-P5 identified PEDVPT-P96 as a potential live-attenuated vaccine candidate. ? 2017 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
complementary DNA; immunoglobulin A; immunoglobulin G; neutralizing antibody; immunoglobulin A; immunoglobulin G; membrane protein; neutralizing antibody; viral protein; virus antibody; animal experiment; animal model; antibody detection; antibody response; antibody titer; Article; comparative study; controlled study; DNA synthesis; freeze thawing; gene sequence; genotype; immune response; immunocytochemistry; immunofluorescence test; immunogenicity; nonhuman; open reading frame; pathogenicity; pig; porcine epidemic diarrhea; Porcine epidemic diarrhea virus; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA extraction; Taiwan; virus shedding; virus strain; amino acid substitution; animal; antibody production; blood; cell culture technique; Chlorocebus aethiops; classification; diarrhea; feces; genetics; genotype; immunology; Porcine epidemic diarrhea virus; sequence alignment; sequence analysis; swine disease; Vero cell line; virology; virulence; whole genome sequencing; Amino Acid Substitution; Animals; Antibodies, Neutralizing; Antibodies, Viral; Antibody Formation; Cell Culture Techniques; Cercopithecus aethiops; Diarrhea; DNA, Complementary; Feces; Genotype; Immunoglobulin A; Immunoglobulin G; Membrane Proteins; Porcine epidemic diarrhea virus; Sequence Alignment; Sequence Analysis; Sus scrofa; Swine; Swine Diseases; Taiwan; Vero Cells; Viral Nonstructural Proteins; Viral Proteins; Virulence; Virus Shedding; Whole Genome Sequencing
SDGs
Other Subjects
complementary DNA; immunoglobulin A; immunoglobulin G; neutralizing antibody; immunoglobulin A; immunoglobulin G; membrane protein; neutralizing antibody; viral protein; virus antibody; animal experiment; animal model; antibody detection; antibody response; antibody titer; Article; comparative study; controlled study; DNA synthesis; freeze thawing; gene sequence; genotype; immune response; immunocytochemistry; immunofluorescence test; immunogenicity; nonhuman; open reading frame; pathogenicity; pig; porcine epidemic diarrhea; Porcine epidemic diarrhea virus; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA extraction; Taiwan; virus shedding; virus strain; amino acid substitution; animal; antibody production; blood; cell culture technique; Chlorocebus aethiops; classification; diarrhea; feces; genetics; genotype; immunology; Porcine epidemic diarrhea virus; sequence alignment; sequence analysis; swine disease; Vero cell line; virology; virulence; whole genome sequencing; Amino Acid Substitution; Animals; Antibodies, Neutralizing; Antibodies, Viral; Antibody Formation; Cell Culture Techniques; Cercopithecus aethiops; Diarrhea; DNA, Complementary; Feces; Genotype; Immunoglobulin A; Immunoglobulin G; Membrane Proteins; Porcine epidemic diarrhea virus; Sequence Alignment; Sequence Analysis; Sus scrofa; Swine; Swine Diseases; Taiwan; Vero Cells; Viral Nonstructural Proteins; Viral Proteins; Virulence; Virus Shedding; Whole Genome Sequencing
Type
journal article