https://scholars.lib.ntu.edu.tw/handle/123456789/573248
標題: | Evaluation and comparison of the pathogenicity and host immune responses induced by a G2b Taiwan porcine epidemic diarrhea virus (strain pintung 52) and its highly cell-culture passaged strain in conventional 5-week-old pigs | 作者: | Chang Y.-C Kao C.-F Chang C.-Y Jeng C.-R Tsai P.-S Pang V.F Chiou H.-Y Peng J.-Y Cheng I.-C IVAN-CHEN CHENG PEI-SHIUE TSAI YEN-CHEN CHANG HUI-WEN CHANG VICTOR FEI PANG Jeng CR |
關鍵字: | complementary DNA; immunoglobulin A; immunoglobulin G; neutralizing antibody; immunoglobulin A; immunoglobulin G; membrane protein; neutralizing antibody; viral protein; virus antibody; animal experiment; animal model; antibody detection; antibody response; antibody titer; Article; comparative study; controlled study; DNA synthesis; freeze thawing; gene sequence; genotype; immune response; immunocytochemistry; immunofluorescence test; immunogenicity; nonhuman; open reading frame; pathogenicity; pig; porcine epidemic diarrhea; Porcine epidemic diarrhea virus; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA extraction; Taiwan; virus shedding; virus strain; amino acid substitution; animal; antibody production; blood; cell culture technique; Chlorocebus aethiops; classification; diarrhea; feces; genetics; genotype; immunology; Porcine epidemic diarrhea virus; sequence alignment; sequence analysis; swine disease; Vero cell line; virology; virulence; whole genome sequencing; Amino Acid Substitution; Animals; Antibodies, Neutralizing; Antibodies, Viral; Antibody Formation; Cell Culture Techniques; Cercopithecus aethiops; Diarrhea; DNA, Complementary; Feces; Genotype; Immunoglobulin A; Immunoglobulin G; Membrane Proteins; Porcine epidemic diarrhea virus; Sequence Alignment; Sequence Analysis; Sus scrofa; Swine; Swine Diseases; Taiwan; Vero Cells; Viral Nonstructural Proteins; Viral Proteins; Virulence; Virus Shedding; Whole Genome Sequencing | 公開日期: | 2017 | 卷: | 9 | 期: | 5 | 來源出版物: | Viruses | 摘要: | A genogroup 2b (G2b) porcine epidemic diarrhea virus (PEDV) Taiwan Pintung 52 (PEDVPT) strain was isolated in 2014. The pathogenicity and host antibody responses elicited by low-passage (passage 5; PEDVPT-P5) and high-passage (passage 96; PEDVPT-P96) PEDVPT strains were compared in post-weaning PEDV-seronegative pigs by oral inoculation. PEDVPT-P5- inoculation induced typical diarrhea during 1-9 days post inoculation with fecal viral shedding persisting for 26 days. Compared to PEDVPT-P5, PEDVPT-P96 inoculation induced none-to-mild diarrhea and lower, delayed fecal viral shedding. Although PEDVPT-P96 elicited slightly lower neutralizing antibodies and PEDV-specific immunoglobulin G (IgG) and immunoglobulin A (IgA) titers, a reduction in pathogenicity and viral shedding of the subsequent challenge with PEDVPT-P5 were noted in both PEDVPT-P5- and PEDVPT-P96-inoculated pigs. Alignment and comparison of full-length sequences of PEDVPT-P5 and PEDVPT-P96 revealed 23 nucleotide changes and resultant 19 amino acid substitutions in non-structure proteins 2, 3, 4, 9, 14, 15, spike, open reading frame 3 (ORF3), and membrane proteins with no detectable deletion or insertion. The present study confirmed the pathogenicity of the PEDVPT isolate in conventional post-weaning pigs. Moreover, data regarding viral attenuation and potency of induced antibodies against PEDVPT-P5 identified PEDVPT-P96 as a potential live-attenuated vaccine candidate. ? 2017 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85019547257&doi=10.3390%2fv9050121&partnerID=40&md5=1e827d1976b09a98eef2b345f8451bea https://scholars.lib.ntu.edu.tw/handle/123456789/573248 |
ISSN: | 19994915 | DOI: | 10.3390/v9050121 | SDG/關鍵字: | complementary DNA; immunoglobulin A; immunoglobulin G; neutralizing antibody; immunoglobulin A; immunoglobulin G; membrane protein; neutralizing antibody; viral protein; virus antibody; animal experiment; animal model; antibody detection; antibody response; antibody titer; Article; comparative study; controlled study; DNA synthesis; freeze thawing; gene sequence; genotype; immune response; immunocytochemistry; immunofluorescence test; immunogenicity; nonhuman; open reading frame; pathogenicity; pig; porcine epidemic diarrhea; Porcine epidemic diarrhea virus; real time polymerase chain reaction; reverse transcription polymerase chain reaction; RNA extraction; Taiwan; virus shedding; virus strain; amino acid substitution; animal; antibody production; blood; cell culture technique; Chlorocebus aethiops; classification; diarrhea; feces; genetics; genotype; immunology; Porcine epidemic diarrhea virus; sequence alignment; sequence analysis; swine disease; Vero cell line; virology; virulence; whole genome sequencing; Amino Acid Substitution; Animals; Antibodies, Neutralizing; Antibodies, Viral; Antibody Formation; Cell Culture Techniques; Cercopithecus aethiops; Diarrhea; DNA, Complementary; Feces; Genotype; Immunoglobulin A; Immunoglobulin G; Membrane Proteins; Porcine epidemic diarrhea virus; Sequence Alignment; Sequence Analysis; Sus scrofa; Swine; Swine Diseases; Taiwan; Vero Cells; Viral Nonstructural Proteins; Viral Proteins; Virulence; Virus Shedding; Whole Genome Sequencing |
顯示於: | 分子暨比較病理生物學研究所 |
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