Arginine vasopressin modulates ion and acid/base balance by regulating cell numbers of sodium chloride cotransporter and h+-atpase rich ionocytes
Journal
International Journal of Molecular Sciences
Journal Volume
21
Journal Issue
11
Pages
3957
Date Issued
2020
Author(s)
Abstract
Arginine vasopressin (Avp) is a conserved pleiotropic hormone that is known to regulate both water reabsorption and ion balance; however, many of the mechanisms underlying its effects remain unclear. Here, we used zebrafish embryos to investigate how Avp modulates ion and acid– base homeostasis. After incubating embryos in double-deionized water for 24 h, avp mRNA expression levels were significantly upregulated. Knockdown of Avp protein expression by an antisense morpholino oligonucleotide (MO) reduced the expression of ionocyte-related genes and downregulated whole-body Cl- content and H+ secretion, while Na+ and Ca2+ levels were not affected. Incubation of Avp antagonist SR49059 also downregulated the mRNA expression of sodium chloride cotransporter 2b (ncc2b), which is a transporter responsible for Cl- uptake. Correspondingly, avp morphants showed lower NCC and H+-ATPase rich (HR) cell numbers, but Na+/K+-ATPase rich (NaR) cell numbers remained unchanged. avp MO also downregulated the numbers of foxi3a-and p63-expressing cells. Finally, the mRNA expression levels of calcitonin gene-related peptide (cgrp) and its receptor, calcitonin receptor-like 1 (crlr1), were downregulated in avp morphants, suggesting that Avp might affect Cgrp and Crlr1 for modulating Cl- balance. Together, our results reveal a molecular/cellular pathway through which Avp regulates ion and acid–base balance, providing new insights into its function. ? 2020 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
adenosine triphosphatase (potassium sodium); antisense oligonucleotide; argipressin; calcitonin gene related peptide; calcitonin gene related peptide receptor; calcitonin receptor like receptor; cystic fibrosis transmembrane conductance regulator; isotocin; messenger RNA; nelivaptan; prolactin; protein p63; proton transporting adenosine triphosphatase; relcovaptan; sodium chloride cotransporter; sodium ion; tolvaptan; antisense oligonucleotide; argipressin; calcitonin gene related peptide; calcium; chloride; complementary DNA; ion; messenger RNA; sodium; sodium chloride cotransporter; vasopressin derivative; zebrafish protein; acid base balance; animal tissue; Article; brain tissue; cell count; controlled study; down regulation; embryo; epithelium cell; gene knockdown; gill; heart tissue; ion transport; ionocyte; kidney tissue; mRNA expression level; muscle tissue; nonhuman; osmoregulation; protein expression; salinity; zebra fish; animal; chemistry; electrode; genetics; homeostasis; in situ hybridization; ion transport; metabolism; skin; Acid-Base Equilibrium; Animals; Arginine Vasopressin; Calcitonin Gene-Related Peptide; Calcium; Chlorides; DNA, Complementary; Down-Regulation; Electrodes; Homeostasis; In Situ Hybridization; Ion Transport; Ions; Oligonucleotides, Antisense; RNA, Messenger; Skin; Sodium; Sodium Chloride Symporters; Vasopressins; Zebrafish; Zebrafish Proteins
Type
journal article