https://scholars.lib.ntu.edu.tw/handle/123456789/581545
標題: | Arginine vasopressin modulates ion and acid/base balance by regulating cell numbers of sodium chloride cotransporter and h+-atpase rich ionocytes | 作者: | Tong S.-K Lee H.-L Lee Y.-C Wu L.-C Tsou Y.-L Lu S.-W Shih S.-W Hwang P.-P Chou M.-Y. MING-YI CHOU |
關鍵字: | adenosine triphosphatase (potassium sodium); antisense oligonucleotide; argipressin; calcitonin gene related peptide; calcitonin gene related peptide receptor; calcitonin receptor like receptor; cystic fibrosis transmembrane conductance regulator; isotocin; messenger RNA; nelivaptan; prolactin; protein p63; proton transporting adenosine triphosphatase; relcovaptan; sodium chloride cotransporter; sodium ion; tolvaptan; antisense oligonucleotide; argipressin; calcitonin gene related peptide; calcium; chloride; complementary DNA; ion; messenger RNA; sodium; sodium chloride cotransporter; vasopressin derivative; zebrafish protein; acid base balance; animal tissue; Article; brain tissue; cell count; controlled study; down regulation; embryo; epithelium cell; gene knockdown; gill; heart tissue; ion transport; ionocyte; kidney tissue; mRNA expression level; muscle tissue; nonhuman; osmoregulation; protein expression; salinity; zebra fish; animal; chemistry; electrode; genetics; homeostasis; in situ hybridization; ion transport; metabolism; skin; Acid-Base Equilibrium; Animals; Arginine Vasopressin; Calcitonin Gene-Related Peptide; Calcium; Chlorides; DNA, Complementary; Down-Regulation; Electrodes; Homeostasis; In Situ Hybridization; Ion Transport; Ions; Oligonucleotides, Antisense; RNA, Messenger; Skin; Sodium; Sodium Chloride Symporters; Vasopressins; Zebrafish; Zebrafish Proteins | 公開日期: | 2020 | 卷: | 21 | 期: | 11 | 起(迄)頁: | 3957 | 來源出版物: | International Journal of Molecular Sciences | 摘要: | Arginine vasopressin (Avp) is a conserved pleiotropic hormone that is known to regulate both water reabsorption and ion balance; however, many of the mechanisms underlying its effects remain unclear. Here, we used zebrafish embryos to investigate how Avp modulates ion and acid– base homeostasis. After incubating embryos in double-deionized water for 24 h, avp mRNA expression levels were significantly upregulated. Knockdown of Avp protein expression by an antisense morpholino oligonucleotide (MO) reduced the expression of ionocyte-related genes and downregulated whole-body Cl- content and H+ secretion, while Na+ and Ca2+ levels were not affected. Incubation of Avp antagonist SR49059 also downregulated the mRNA expression of sodium chloride cotransporter 2b (ncc2b), which is a transporter responsible for Cl- uptake. Correspondingly, avp morphants showed lower NCC and H+-ATPase rich (HR) cell numbers, but Na+/K+-ATPase rich (NaR) cell numbers remained unchanged. avp MO also downregulated the numbers of foxi3a-and p63-expressing cells. Finally, the mRNA expression levels of calcitonin gene-related peptide (cgrp) and its receptor, calcitonin receptor-like 1 (crlr1), were downregulated in avp morphants, suggesting that Avp might affect Cgrp and Crlr1 for modulating Cl- balance. Together, our results reveal a molecular/cellular pathway through which Avp regulates ion and acid–base balance, providing new insights into its function. ? 2020 by the authors. Licensee MDPI, Basel, Switzerland. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-85085757592&doi=10.3390%2fijms21113957&partnerID=40&md5=cca02644bd02078ecff6d502d862ec8f https://scholars.lib.ntu.edu.tw/handle/123456789/581545 |
DOI: | 10.3390/ijms21113957 |
顯示於: | 生命科學系 |
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