Expression of SPLUNC1 protein in nasal polyp epithelial cells in air-liquid interface culture treated with IL-13
Journal
American Journal of Rhinology and Allergy
Journal Volume
24
Journal Issue
1
Pages
17-20
Date Issued
2010
Author(s)
Abstract
Background: Short palate, lung, and nasal epithelium clone 1 (SPLUNC1) protein is an airway epithelial cell - derived molecule exerting host defense against pathogen. However, the function and regulation of SPLUNC1 in nasal epithelial cells are still unclear. Chronic rhinosinusitis with nasal polyps (CRSwNPs) is a disorder characterized by eosinophilic Th2 inflammation and frequent microbial colonization. The pathogenesis has been postulated as a disturbed mucosal immune response. This study investigates the SPLUNC1 expression of nasal polyp epithelial cells in air-liquid interface (ALI) culture and after treating with Th2 inflammatory cytokines IL-13. Methods: Human nasal polyp epithelial cells isolated from patients with CRSwNPs were put in different cell culture models at days 0 and 21 and were assessed for expression of SPLUNC1 by microarray. Cultured cells in ALI plus retinoic acid (ALI + RA) model were then incubated with 0, 1, 10, and 100 ng/mL human recombinant IL-13 for up to 5 days. The expression of SPLUNC1 was assessed by real-time quantitative polymerase chain reaction (RT-Q-PCR), reverse-transcriptase PCR (RT-PCR) and Western blot analysis. Results: ALI + RA culture model harvesting ciliary differentiated nasal epithelial cells constitutively expressed high levels of SPLUNC1. In contrast, SPLUNC1 is reduced under classic submerged single layer culture. SPLUNC1 is also dose-responsively down-regulated after incubation with IL-13. Conclusion: A microenvironmental milieu containing IL-13 may be detrimental to the host innate immunity response, at least in part, through the inhibition of SPLUNC1 production. Copyright ? 2010, OceanSide Publications, Inc., U.S.A.
SDGs
Other Subjects
cell membrane protein; interleukin 13; recombinant cytokine; retinol acetate; short palate lung and nasal epithelium clone 1; unclassified drug; article; cell culture; cell differentiation; ciliated epithelium; clinical article; clinical trial; concentration response; controlled study; down regulation; epithelium cell; human; human cell; innate immunity; microarray analysis; monolayer culture; nose polyp; nucleotide sequence; protein expression; protein synthesis inhibition; quantitative analysis; real time polymerase chain reaction; reverse transcription polymerase chain reaction; submerged cell culture; Th2 cell; Western blotting; Cell Culture Techniques; Cells, Cultured; Chronic Disease; Dose-Response Relationship, Immunologic; Down-Regulation; Epithelial Cells; Glycoproteins; Humans; Interleukin-13; Microarray Analysis; Nasal Polyps; Phosphoproteins; Rhinitis; Sinusitis; Tretinoin
Type
journal article