https://scholars.lib.ntu.edu.tw/handle/123456789/592677
標題: | Expression of SPLUNC1 protein in nasal polyp epithelial cells in air-liquid interface culture treated with IL-13 | 作者: | TE-HUEI YEH Lee, Shiann-Yann WEI-CHUNG HSU |
公開日期: | 2010 | 卷: | 24 | 期: | 1 | 起(迄)頁: | 17-20 | 來源出版物: | American Journal of Rhinology and Allergy | 摘要: | Background: Short palate, lung, and nasal epithelium clone 1 (SPLUNC1) protein is an airway epithelial cell - derived molecule exerting host defense against pathogen. However, the function and regulation of SPLUNC1 in nasal epithelial cells are still unclear. Chronic rhinosinusitis with nasal polyps (CRSwNPs) is a disorder characterized by eosinophilic Th2 inflammation and frequent microbial colonization. The pathogenesis has been postulated as a disturbed mucosal immune response. This study investigates the SPLUNC1 expression of nasal polyp epithelial cells in air-liquid interface (ALI) culture and after treating with Th2 inflammatory cytokines IL-13. Methods: Human nasal polyp epithelial cells isolated from patients with CRSwNPs were put in different cell culture models at days 0 and 21 and were assessed for expression of SPLUNC1 by microarray. Cultured cells in ALI plus retinoic acid (ALI + RA) model were then incubated with 0, 1, 10, and 100 ng/mL human recombinant IL-13 for up to 5 days. The expression of SPLUNC1 was assessed by real-time quantitative polymerase chain reaction (RT-Q-PCR), reverse-transcriptase PCR (RT-PCR) and Western blot analysis. Results: ALI + RA culture model harvesting ciliary differentiated nasal epithelial cells constitutively expressed high levels of SPLUNC1. In contrast, SPLUNC1 is reduced under classic submerged single layer culture. SPLUNC1 is also dose-responsively down-regulated after incubation with IL-13. Conclusion: A microenvironmental milieu containing IL-13 may be detrimental to the host innate immunity response, at least in part, through the inhibition of SPLUNC1 production. Copyright ? 2010, OceanSide Publications, Inc., U.S.A. |
URI: | https://www.scopus.com/inward/record.uri?eid=2-s2.0-75749090787&doi=10.2500%2fajra.2010.24.3381&partnerID=40&md5=33b98eef1497712f15ea42e9de5304e9 https://scholars.lib.ntu.edu.tw/handle/123456789/592677 |
ISSN: | 1945-8924 | DOI: | 10.2500/ajra.2010.24.3381 | SDG/關鍵字: | cell membrane protein; interleukin 13; recombinant cytokine; retinol acetate; short palate lung and nasal epithelium clone 1; unclassified drug; article; cell culture; cell differentiation; ciliated epithelium; clinical article; clinical trial; concentration response; controlled study; down regulation; epithelium cell; human; human cell; innate immunity; microarray analysis; monolayer culture; nose polyp; nucleotide sequence; protein expression; protein synthesis inhibition; quantitative analysis; real time polymerase chain reaction; reverse transcription polymerase chain reaction; submerged cell culture; Th2 cell; Western blotting; Cell Culture Techniques; Cells, Cultured; Chronic Disease; Dose-Response Relationship, Immunologic; Down-Regulation; Epithelial Cells; Glycoproteins; Humans; Interleukin-13; Microarray Analysis; Nasal Polyps; Phosphoproteins; Rhinitis; Sinusitis; Tretinoin |
顯示於: | 醫學院附設醫院 (臺大醫院) |
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