Enhancement of rotator cuff healing with farnesol-impregnated gellan gum/hyaluronic acid hydrogel membranes in a rabbit model
Journal
Pharmaceutics
Journal Volume
13
Journal Issue
7
Date Issued
2021
Author(s)
Abstract
Most rotator cuff (RC) tears occur at the bone–tendon interface and cause disability and pain. Farnesol, a sesquiterpene compound, can exert antioxidative and anti-inflammatory effects and promote collagen synthesis. In this rabbit model, either commercial SurgiWrap membrane or hydrogel membranes containing various compositions of gellan gum, hyaluronic acid, and farnesol (hereafter GHF membranes) were applied to the tear site, and the repair of the cuff was examined 2 and 3 weeks afterward. The designed membranes swelled rapidly and adsorbed onto the tear site more readily and closely than the SurgiWrap membrane. The membranes degraded slowly and functioned as both a barrier and a vehicle of slow farnesol release during the repair period. Farnesol enhanced collagen production in myoblasts and tenocytes, and interleukin 6 and tumor necrosis factor α levels were modulated. Gross observations and histological examinations indicated that the GHF membranes impregnated with 4 mM farnesol resulted in superior RC repair. In sum, the slow release of farnesol from hydrogel membranes can be beneficial in the repair of RC injuries. ? 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Subjects
Collagen synthesis
Farnesol
Hyaluronan
Hydrogel membranes
Rotator cuff tendon tears
farnesol
gellan
hyaluronic acid
hydrogel
interleukin 6
tumor necrosis factor
animal experiment
animal model
animal tissue
antioxidant assay
Article
biocompatibility
bone regeneration
cell migration
cell viability
collagen fiber density
collagen fiber orientation
controlled study
cytotoxicity
drug release
extracellular matrix
fluorescence microscopy
histology
histopathology
Leporidae
male
MTT assay
myoblast
nonhuman
optical coherence tomography
osseointegration
rotator cuff
rotator cuff injury
rotator cuff rupture
scanning electron microscopy
swelling test
tendon bone interface
tenocyte
tissue engineering
tissue regeneration
water content
SDGs
Type
journal article