Microarrays for the scalable production of uniform and metabolically relevant tumour spheroids
Journal
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Journal Volume
3
ISBN
9781618390622
Date Issued
2010-12-01
Author(s)
Hardelauf, H.
Frimat, J. P.
Schormann, W.
Stewart, J. D.
Cadenas, C.
Franzke, J.
Hengstler, J. G.
Kunz-Schughart, L. A.
West, J.
Abstract
A microarray printing technique was used for the mass production of highly uniform human HT29 colon carcinoma spheroids. The spheroids were truly spherical and had organotypic densities. The array pitch impacted growth kinetics and was used to tune the spheroid diameter from 200 to 550 μm. During sustained culture mass transfer gradients were established, reflecting the microenvironment of avascularised tumours. The spheroids have reduced chemosensitivity towards the anti-cancer agent irinotecan, with further reduced sensitivity following continued growth into a hypoxic phase of culture. In summary, microarray-based spheroid culture has great potential to support drug discovery and tumour biology research.
Subjects
Chemosensitivity | Irinotecan | Metabolic gradients | Microarray | Tumour spheroids
Type
conference paper