Electrical detection of protein using gold nanoparticles and nanogap electrodes
Journal
Japanese Journal of Applied Physics
Journal Volume
44
Journal Issue
7 B
Pages
5711-5716
Date Issued
2005
Author(s)
Abstract
A method of electrically detecting of protein described is developed using self-assembled multilayer gold nanoparticles (AuNPs) on a SiO2/Si substrate between gold electrodes. Electrical measurements are performed at room temperature using a probe station. A monoclonal antibody is immobilized on the top surface of the first layer of AuNPs (14nm). The second layer of AuNPs is formed through specific binding among a target antigen [hepatitis C virus, (HCV)], the monoclonal antibody, and the conjugate of a AuNP-polyclonal antibody. Once the specific binding among the monoclonal antibody, target antigen, and polyclonal antibody occurs, a significant electric current is detected through multilayer self-assembled gold nanoparticles between nanogap electrodes. No significant current (<1 pA) can be measured through a monolayer of AuNPs. A significant difference between the IV curves of the monolayer and the multilayer of AuNPs is used to identify whether the target antigen exists in the tested sample. ? 2005 The Japan Society of Applied Physics.
Subjects
Electrical detection; Gold nanoparticle; Multilayer; Protein chip; Self-assembled monolayer
SDGs
Other Subjects
Gold; Monolayers; Nanostructured materials; Self assembly; Substrates; Electrical detection; Gold nanoparticles; Protein chip; Proteins
Type
journal article