行政院國家科學委員會專題研究計畫成果報告:水禽敗血症巴氏桿菌AROA基因變異株之建立及抗原性分析(II)

Pasteurella multocida (PM) and
Riemerella anatipestifer (RA) infection,
commonly known as fowl cholera and
infectious serositis in waterfowl, continue to
be a significant problem. Vaccines against
both diseases are available but their efficacy
has been poor. Most bacterins containing
one or several serotypes of formalininactivated
bacteria have induced protection.
The bacterins also have caused transient
endotoxin shock. The approach of
attenuation based on several auxotrophic
genes, such as aroA, has been employed to
make live attenuated bacterins. The aroA
gene of PM & RA will be cloned by
complementation of the E. coli mutant after
electroporation with a genomic library
constructed in plasmid. Their sequences
will be determined by Sanger‘s dideoxy
method. The genes will be inactivated by
insertion of a antibiotic resistance gene and
reintroduced by allelic exchange into the
chromosome of the parental PM & RA.
The mutants’ virulence will be examined in a
mouse model.