Preventive Effects of Lactobacillus Mixture against Chronic Kidney Disease Progression through Enhancement of Beneficial Bacteria and Downregulation of Gut-Derived Uremic Toxins
Journal
Journal of Agricultural and Food Chemistry
Journal Volume
69
Journal Issue
26
Pages
7353-7366
Date Issued
2021
Author(s)
Abstract
Gut dysbiosis is a major contributor to adverse chronic kidney disease (CKD) progression, and microbiota-based strategies could be considered as a novel therapeutic and preventative target. In this study, a probiotic screening platform based on gut-derived uremic toxin-reducing probiotics was developed and the underlying mechanism was further verified through a 0.2% adenine-induced CKD mouse model. Two strains (Lactobacillus paracasei and Lactobacillus plantarum) were selected due to their high clearance ability and named Lactobacillus mix (Lm). The results showed that Lm significantly improved the kidney function by reducing kidney injury and fibrotic-related proteins. Furthermore, Lm decreased oxidative stress and proinflammatory reactions and elevated immune responses in the kidney. Importantly, Lm reversed gut dysbiosis and restored the abundance of commensal bacteria, especially short-chain fatty acid producers, leading to improved intestinal barrier integrity via modulation of microbial composition and metabolite production. Taken together, these findings provided evidence that Lm could be a preventive approach against CKD. ? 2021 American Chemical Society.
Subjects
chronic kidney disease
gut dysbiosis
gut-derived uremic toxins
Lactobacillus
probiotic screening platform
Bacilli
Diagnosis
Metabolites
Volatile fatty acids
Chronic kidney disease
Immune response
Intestinal barriers
Lactobacillus plantarum
Metabolite production
Microbial composition
Proinflammatory
Short-chain fatty acids
Probiotics
probiotic agent
animal
bacterium
chronic kidney failure
disease exacerbation
down regulation
intestine flora
mouse
Animals
Bacteria
Disease Progression
Down-Regulation
Gastrointestinal Microbiome
Mice
Renal Insufficiency, Chronic
SDGs
Type
journal article