Preferential induction of transforming growth factor-β production in gastric epithelial cells and monocytes by Helicobacter pylori soluble proteins
Journal
Journal of Infectious Diseases
Journal Volume
196
Journal Issue
9
Pages
1386-1393
Date Issued
2007-11-01
Author(s)
Ching-Yi Lin
Yuan-Ting Hsieh
Yi-Han Chiu
Kuang-Wen Liao
Abstract
Background. The cytokines induced by Helicobacter pylori, as well as the intricate balance of proinflammatory and anti-inflammatory cytokines, are relevant to the outcomes of H. pylori infection. Transforming growth factor (TGF)-β and interleukin (IL)-10 are 2 vital anti-inflammatory cytokines that regulate mucosal immunity in various inflammatory and infectious diseases. Methods. To elucidate whether host-bacteria interaction can influence TGF-β and IL-10 production, we investigated the expression of TGF-β and IL-10 in various mammalian cell lines preincubated with H. pylori and other enteric bacteria. Results. The amount of TGF-β protein, but not IL-10, was significantly increased after stimulation with H. pylori, but other enteric bacteria did not induce TGF-β production. Different H. pylori strains isolated from patients with gastritis, peptic ulcer, gastric cancer and strains with cagA or vacA isogenic mutations showed similar effects on TGF-β induction, indicating that this effect was a constitutional characteristic of H. pylori and independent of cagA and vacA status. Conclusion. The results imply the presence of a protein factor (termed "TGF-β-inducing protein") that induces production of TGF-β. In view of the multiple effects of TGF-β, we conclude the TGF-β-inducing protein of H. pylori might mediate the immune response and contribute to the pathogenesis of H. pylori infection. ? 2007 by the Infectious Diseases Society of America. All rights reserved.
SDGs
Other Subjects
bacterial protein; interleukin 10; transforming growth factor beta; bacterial antigen; bacterial protein; cagA protein, Helicobacter pylori; interleukin 10; transforming growth factor beta; unclassified drug; VacA protein, Helicobacter pylori; article; bacterium isolation; controlled study; cytokine production; duodenum ulcer; Enterobacteriaceae; gastritis; Helicobacter infection; Helicobacter pylori; human; human cell; major clinical study; monocyte; mutational analysis; priority journal; protein expression; stomach cancer; stomach epithelium; stomach ulcer; biosynthesis; chemistry; cytology; epithelium cell; genetics; Helicobacter pylori; metabolism; monocyte; stomach; tumor cell line; Antigens, Bacterial; Bacterial Proteins; Cell Line, Tumor; Epithelial Cells; Helicobacter pylori; Humans; Interleukin-10; Monocytes; Stomach; Transforming Growth Factor beta
Type
journal article