Ameliorative effects of functional chalaza hydrolysates prepared from protease-A digestion on cognitive dysfunction and brain oxidative damages
Journal
Poultry Science
Journal Volume
99
Journal Issue
5
Pages
2819-2832
Date Issued
2020
Author(s)
Chan, Chia-Jung
Tseng, Jung-Kai
Lin, Yi-Ling
Samuel Wu, Yi-Hsieng
Chen, Jr-Wei
Abstract
Our patented protease A-digested crude chalaza hydrolysates (CCH) show antioxidant abilities in vitro. The prophylactic effects of CCH on cognitive dysfunction and brain oxidative damages were investigated via a D-galactose (DG)-injected mouse model in this study. Fifty-four mice were randomly divided into the following: (1) CON, 0.1 mL 0.9% saline (subcutaneous injection [SC] on the back)+distilled water (oral gavage); (2) DG, 100 mg/kg BW/day D-galactose (Bio-Serv Co., Flemington, NJ, USA) (SC on the back)+distilled water (oral gavage); (3) DG_LCH, 100 mg/kg BW/day D-galactose (SC on the back) + 50 mg CCH/kg BW/day in 0.1 ml distilled water (oral gavage); (4) DG_MCH, 100 mg/kg BW/day D-galactose (SC on the back) + 100 mg CCH/kg BW/day (oral gavage); (5) DG_HCH, 100 mg/kg BW/day D-galactose (SC on the back) + 200 mg CCH/kg BW/day (oral gavage); (6) DG_AG, 100 mg/kg BW/day D-galactose (SC on the back) + 100 mg aminoguanidine hydrochloride/kg BW/day (oral gavage). The experiment lasted for 84 D. CCH, containing antioxidant-free amino acids and anserine, restored (P < 0.05) DG-injected memory injury in the Morris water maze test and attenuated the neuronal degenerations and nucleus shrinkages in the dentate gyrus area. CCH supplementation also reduced amyloid β-peptide protein levels and accumulation of advanced glycation end products (AGE) in the brain of DG-injected mice, whereas the brain antioxidant capacity was reversed (P < 0.05) by supplementing CCH. Furthermore, AGE receptor (RAGE), NFκb, IL-6, and TNF-α gene expressions were downregulated (P < 0.05) by supplementing CCH. Therefore, CCH show prophylactic effects on the development of oxidative stress-induced cognitive dysfunction.
SDGs
Other Subjects
anserine; antiinflammatory agent; antioxidant; carnosine; protective agent; protein hydrolysate; animal; chemistry; chicken; cognitive defect; drug effect; egg yolk; hippocampus; Institute for Cancer Research mouse; learning; male; maze test; memory; metabolism; mouse; nerve cell; oxidative stress; physiology; Animals; Anserine; Anti-Inflammatory Agents; Antioxidants; Carnosine; Chickens; Cognitive Dysfunction; Egg Yolk; Hippocampus; Learning; Male; Maze Learning; Memory; Mice; Mice, Inbred ICR; Neurons; Oxidative Stress; Protective Agents; Protein Hydrolysates
Type
journal article